CAV3 Antibody - N-terminal region (OAAB03503)

Data Sheet
 
Product Number OAAB03503
Product Page www.avivasysbio.com/cav3-antibody-n-terminal-region-oaab03503.html
Name CAV3 Antibody - N-terminal region (OAAB03503)
Molecular Weight 17259 Da
Isotype Rabbit Ig
NCBI Gene Id 859
Host Rabbit
Clonality Polyclonal
Concentration Approximately 0.5mg/ml. Actual concentration varies with each lot.
Alias Symbols LQT9, MPDT, RMD2, VIP21, LGMD1C, VIP-21
Product Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Reconstitution and Storage Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-CAV3 antibody - OAAB03503
Application Info WB: 1:500
WB: 1:1000
IHC-P: 1:10~50
FC: 1:10~50
Other Applications Image 1 Data Flow cytometric analysis of 293 cells using CAV3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunogen This CAV3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-31 amino acids from the N-terminal region of human CAV3.
Uniprot ID P56539
Protein Name Caveolin-3
Publications

Pavlović, D., McLatchie, L. M. & Shattock, M. J. The rate of loss of T-tubules in cultured adult ventricular myocytes is species dependent. Exp. Physiol. 95, 518-27 (2010). 20061354

Sample Type Confirmation

CAV3 is strongly supported by BioGPS gene expression data to be expressed in HEK293

Protein Accession # NP_001225.1
Gene Symbol CAV3
Predicted Species Reactivity Human, Mouse, Rat, Cow, Pig
Application WB, IHC, FC
Predicted Homology Based on Immunogen Sequence Cow; Human; Mouse; Pig; Rat
Image 1
293
Western blot analysis of CAV3 antibody (N-term) in 293 cell lysates (35ug/lane). CAV3 (arrow) was detected using the purified Pab.

CAV3 is strongly supported by BioGPS gene expression data to be expressed in HEK293

Image 2
Human Skeletal Muscle
Formalin-fixed and paraffin-embedded human Skeletal muscle reacted with CAV3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Image 3
293
Flow cytometric analysis of 293 cells using CAV3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
 

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