Product Number |
OAAB03423 |
Product Page |
www.avivasysbio.com/eda-antibody-n-terminal-region-oaab03423.html |
Name |
EDA Antibody - N-terminal region (OAAB03423) |
Molecular Weight |
41294 Da |
Isotype |
Rabbit Ig |
NCBI Gene Id |
1896 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
Approximately 0.5mg/ml. Actual concentration varies with each lot. |
Description |
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Alias Symbols |
ED1, HED, EDA1, EDA2, HED1, ODT1, XHED, ECTD1, XLHED, ED1-A1, ED1-A2, EDA-A1, EDA-A2, TNLG7C, STHAGX1 |
Product Format |
Liquid. PBS with 0.09% (W/V) sodium azide. |
Description of Target |
EDA is a type II membrane protein that can be cleaved by furin to produce a secreted form. This protein, which belongs to the tumor necrosis factor family, acts as a homotrimer and may be involved in cell-cell signaling during the development of ectodermal organs. Defects in the gene for EDA are a cause of ectodermal dysplasia, anhidrotic, which is also known as X-linked hypohidrotic ectodermal dysplasia.
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Reconstitution and Storage |
Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-EDA antibody - OAAB03423 |
Application Info |
WB: 1:1000 IHC-P: 1:10~50 FC: 1:10~50 |
Other Applications Image 1 Data |
Flow cytometric analysis of hela cells using EDA Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Immunogen |
This EDA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 47-76 amino acids from the N-terminal region of human EDA. |
Uniprot ID |
Q92838 |
Protein Name |
Ectodysplasin-A |
Publications |
a de novo 3.8-Mb inversion affecting the EDA and XIST genes in a heterozygous female calf with generalized hypohidrotic ectodermal dysplasia. BMC Genomics. 20, 715 (2019). 31533624 |
Protein Accession # |
NP_001005609.1 |
Purification |
This antibody is purified through a protein A column, followed by peptide affinity purification. |
Gene Symbol |
EDA |
Predicted Species Reactivity |
Human |
Application |
WB, IHC-P, FC |
Predicted Homology Based on Immunogen Sequence |
Bovine |
Image 1 | Hela
| Western blot analysis of anti-EDA Antibody (N-term) (RB13883) in Hela cell lysates (35ug/lane). EDA(arrow) was detected using the purified Pab (1:60 dilution). |
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Image 2 | human hepatocarcinoma
| Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with EDA antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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Image 3 | Hela
| Flow cytometric analysis of hela cells using EDA Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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