| Product Number |
OAAB01459 |
| Product Page |
www.avivasysbio.com/mcam-antibody-center-region-oaab01459.html |
| Name |
MCAM Antibody - middle region (OAAB01459) |
| Molecular Weight |
71607 Da |
| Isotype |
Rabbit Ig |
| NCBI Gene Id |
4162 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
Approximately 0.5mg/ml. Actual concentration varies with each lot. |
| Alias Symbols |
CD146, MUC18, HEMCAM, METCAM, MelCAM |
| Product Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Reconstitution and Storage |
Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-MCAM antibody - OAAB01459 |
| Application Info |
WB: 1:1000
IHC-P: 1:50~100
FC: 1:10~50 |
| Other Applications Image 1 Data |
Flow cytometric analysis of hela cells using MCAM Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
| Immunogen |
This MCAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 161-189 amino acids from the Central region of human MCAM. |
| Uniprot ID |
P43121 |
| Protein Accession # |
NP_006491 |
| Gene Symbol |
MCAM |
| Predicted Species Reactivity |
Human |
| Application |
WB, IHC, FC |
| Predicted Homology Based on Immunogen Sequence |
Human |
| Image 1 | A2058
 | Western blot analysis of MCAM Antibody (Center) in A2058 cell lysates (35ug/lane). MCAM (arrow) was detected using the purified Pab.(2ug/ml) |
| | Image 2 | Hela
 | Flow cytometric analysis of hela cells using MCAM Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
| | Image 3 | Human Skin
 | Formalin-fixed and paraffin-embedded human skin reacted with MCAM Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
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