RAB8A Antibody - middle region (AVARP13076_P050)

Data Sheet
 
Product Number AVARP13076_P050
Product Page www.avivasysbio.com/rab8a-antibody-middle-region-avarp13076-p050.html
Name RAB8A Antibody - middle region (AVARP13076_P050)
Protein Size (# AA) 207 amino acids
Molecular Weight 24kDa
NCBI Gene Id 4218
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name RAB8A, member RAS oncogene family
Alias Symbols MEL, RAB8
Peptide Sequence Synthetic peptide located within the following region: GIKFMETSAKANINVENAFFTLARDIKAKMDKKLEGNSPQGSNQGVKITP
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Roland,J.T., (2007) Mol. Biol. Cell 18 (8), 2828-2837
Description of Target RAB8A is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable.The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Protein Interactions TFRC; SYTL4; SYTL1; TBC1D17; STAU1; UBC; MDM2; RPA3; RPA2; RPA1; STK4; OPTN; Myo5b; MYO5C; EHD3; EHD1; UBL4A; RAB1B; RAB31; COX5A; RAB11B; RAB7A; SCP2; RRBP1; NDUFA7; IDH3G; SNCA; ELAVL1; Rab8a; RPH3A; RAB3IP; RIMS2; RPH3AL; RABIF; MAP4K2; PDE6D; GDI2; OC
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-RAB8A (AVARP13076_P050) antibody
Blocking Peptide For anti-RAB8A (AVARP13076_P050) antibody is Catalog # AAP30723 (Previous Catalog # AAPP01380)
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human RAB8A
Uniprot ID P61006
Protein Name Ras-related protein Rab-8A
Sample Type Confirmation

RAB8A is supported by BioGPS gene expression data to be expressed in 721_B

Protein Accession # NP_005361
Purification Affinity Purified
Nucleotide Accession # NM_005370
Tested Species Reactivity Human
Gene Symbol RAB8A
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Pig, Sheep, Zebrafish
Application WB
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Human: 100%; Mouse: 85%; Pig: 100%; Rat: 85%; Sheep: 100%; Zebrafish: 85%
Image 1
Human, Monkey
RAB8A antibody - middle region (AVARP13076_P050) validated by WB using 1. Human Cervical Cancer Cell Lysate (15ug)
2. Monkey Fibroblast Cell Lysate (15ug)
3. Human Cervical Cancer Cell transfected with mouse Rab8A-GFP (15ug) at 1:1000.
Image 2
Human Fetal Liver
Host: Rabbit
Target Name: RAB8A
Sample Type: Human Fetal Liver
Antibody Dilution: 1.0ug/ml
Image 3
Human 721_B
WB Suggested Anti-RAB8A Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: 721_B cell lysateRAB8A is supported by BioGPS gene expression data to be expressed in 721_B
 

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