Product Number |
AVARP13076_P050 |
Product Page |
www.avivasysbio.com/rab8a-antibody-middle-region-avarp13076-p050.html |
Name |
RAB8A Antibody - middle region (AVARP13076_P050) |
Protein Size (# AA) |
207 amino acids |
Molecular Weight |
24kDa |
NCBI Gene Id |
4218 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
RAB8A, member RAS oncogene family |
Alias Symbols |
MEL, RAB8 |
Peptide Sequence |
Synthetic peptide located within the following region: GIKFMETSAKANINVENAFFTLARDIKAKMDKKLEGNSPQGSNQGVKITP |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Roland,J.T., (2007) Mol. Biol. Cell 18 (8), 2828-2837 |
Description of Target |
RAB8A is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable.The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
Protein Interactions |
TFRC; SYTL4; SYTL1; TBC1D17; STAU1; UBC; MDM2; RPA3; RPA2; RPA1; STK4; OPTN; Myo5b; MYO5C; EHD3; EHD1; UBL4A; RAB1B; RAB31; COX5A; RAB11B; RAB7A; SCP2; RRBP1; NDUFA7; IDH3G; SNCA; ELAVL1; Rab8a; RPH3A; RAB3IP; RIMS2; RPH3AL; RABIF; MAP4K2; PDE6D; GDI2; OC |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-RAB8A (AVARP13076_P050) antibody |
Blocking Peptide |
For anti-RAB8A (AVARP13076_P050) antibody is Catalog # AAP30723 (Previous Catalog # AAPP01380) |
Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human RAB8A |
Uniprot ID |
P61006 |
Protein Name |
Ras-related protein Rab-8A |
Sample Type Confirmation |
RAB8A is supported by BioGPS gene expression data to be expressed in 721_B |
Protein Accession # |
NP_005361 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_005370 |
Tested Species Reactivity |
Human |
Gene Symbol |
RAB8A |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Pig, Sheep, Zebrafish |
Application |
WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Human: 100%; Mouse: 85%; Pig: 100%; Rat: 85%; Sheep: 100%; Zebrafish: 85% |
Image 1 | Human, Monkey
| RAB8A antibody - middle region (AVARP13076_P050) validated by WB using 1. Human Cervical Cancer Cell Lysate (15ug) 2. Monkey Fibroblast Cell Lysate (15ug) 3. Human Cervical Cancer Cell transfected with mouse Rab8A-GFP (15ug) at 1:1000. |
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Image 2 | Human Fetal Liver
| Host: Rabbit Target Name: RAB8A Sample Type: Human Fetal Liver Antibody Dilution: 1.0ug/ml |
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Image 3 | Human 721_B
| WB Suggested Anti-RAB8A Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive Control: 721_B cell lysateRAB8A is supported by BioGPS gene expression data to be expressed in 721_B |
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