NFKBIA Antibody - middle region (AVARP09044_P050)

• Product Number: AVARP09044_P050
• Name: NFKBIA Antibody - middle region (AVARP09044_P050)
• Protein Size (# AA): 317 amino acids
• Molecular Weight: 35kDa
• NCBI Gene Id: 4792
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• Alias Symbols: IKBA, MAD-3, NFKBI, EDAID2
• Peptide Sequence: Synthetic peptide located within the following region: SPYQLTWGRPSTRIQQQLGQLTLENLQMLPESEDEESYDTESEFTEFTED
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Truhlar,S.M., (2008) J. Mol. Biol. 380 (1), 67-82
• Description of Target: NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine). NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Protein Interactions: BTRC; NEDD9; IKBKB; CHUK; UBC; MTOR; IKBKG; SKP1; FBXW11; UBE2D3; RELA; NPLOC4; VCP; UFD1L; NFKB1; TNF; SUMO1; UBE2D1; COPS3; GPS1; SUMO3; Csnk2b; PRKCA; ST7; STAT1; REL; POLR2C; CD7; ARRB2; ARRB1; SUMO2; PSMA1; ZNF212; IKZF4; ATF4; TBK1; IKBKE; DNAJA3; S
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-NFKBIA (AVARP09044_P050) antibody is Catalog # AAP30582 (Previous Catalog # AAPP01234)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NFKBIA
• Uniprot ID: P25963
• Protein Name: NF-kappa-B inhibitor alpha
• Protein Accession #: NP_065390
• Purification: Affinity Purified
• Nucleotide Accession #: NM_020529
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: NFKBIA
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Sheep
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rat: 100%; Sheep: 100%
• Image 1: Human 293T
  
  WB Suggested Anti-NFKBIA Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:62500
  Positive Control: 293T cell lysate
• Image 2: Mouse
  
  Lanes:
  1. 100 ug mouse heart lysate
  2. 100 ug mouse kidney lysate
  3. 100 ug mouse lung lysate
  4. 100 ug mouse thymus lysate
  5. 100 ug mouse spleen lysate
  Primary Antibody Dilution:
  1:1000
  Secondary Antibody:
  Anti-rabbit-AP
  Secondary Antibody Dilution:
  1:10000
  Gene Name:
  NFKBIA
  Submitted by:
  Andreia Carvalho, Instituto de Biologia Molecular e Celular, Universidade do Porto (IBMC-UP)/Organelle Biogenesis and Function (OBF) Group