Product Number |
AVARP06007_P050 |
Product Page |
www.avivasysbio.com/mdm4-antibody-n-terminal-region-avarp06007-p050.html |
Name |
MDM4 Antibody - N-terminal region (AVARP06007_P050) |
Protein Size (# AA) |
490 amino acids |
Molecular Weight |
55kDa |
NCBI Gene Id |
4194 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Mdm4 p53 binding protein homolog (mouse) |
Alias Symbols |
HDMX, MDMX, MRP1, BMFS6 |
Peptide Sequence |
Synthetic peptide located within the following region: IPSQDQLKQSAEESSTSRKRTTEDDIPTLPTSEHKCIHSREDEDLIENLA |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Danovi,D., et al., (2004) Mol. Cell. Biol. 24 (13), 5835-5843 |
Description of Target |
MDM4 inhibits p53- and p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. It inhibits degradation of MDM2. It can reverse MDM2-targeted degradation of p53 while maintaining suppression of p53 transactivation and apoptotic functions. The human MDM4 gene, which plays a role in apoptosis, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal. The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein. The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested. MDM4 protein produced by in vitro translation interacts with p53 via a binding domain located in the N-terminal region of the MDM4 protein. MDM4 shows significant structural similarity to p53-binding protein MDM2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
Protein Interactions |
RNF115; CAPN7; CD160; MDM2; TP53; UBC; MDM4; USP2; YWHAG; UBE2D2; PRKAA1; BCL2; RPS20; RPS15; RPL37; YWHAQ; HSP90AA1; CSNK1A1; PPP1CC; PPP1CB; PPP1CA; RB1; APP; UBE2D3; UBE2D1; MNAT1; ABL1; LRSAM1; TRIM55; TRIM46; MKRN3; YWHAZ; YWHAE; YWHAB; CDKN1A; RFWD2 |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-MDM4 (AVARP06007_P050) antibody |
Blocking Peptide |
For anti-MDM4 (AVARP06007_P050) antibody is Catalog # AAPS31602 |
Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human MDM4 |
Uniprot ID |
O15151 |
Protein Name |
Protein Mdm4 |
Publications |
Schlaeger, C. et al. Etiology-dependent molecular mechanisms in human hepatocarcinogenesis. Hepatology 47, 511-20 (2008). 18161050 |
Protein Accession # |
NP_002384 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_002393 |
Tested Species Reactivity |
Human |
Gene Symbol |
MDM4 |
Predicted Species Reactivity |
Human, Cow, Dog, Horse, Pig, Rabbit |
Application |
WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 80%; Dog: 93%; Horse: 93%; Human: 100%; Pig: 86%; Rabbit: 80% |
Image 1 | Human HepG2
| WB Suggested Anti-MDM4 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive Control: HepG2 cell lysate |
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Image 2 | Human MCF-7
| Lanes: 1. 10 ug MCF7 cell lysate 2. 10 ug MCF7 cell lysate 3. 10 ug MCF7 cell lysate 4. 10 ug MCF7 cell lysate Primary Antibody Dilution: 1:1000 Secondary Antibody: Anti-Rabbit HRP Secondary Antibody Dilution: 1:10,000 Gene Name: MDM4 Submitted by: Anonymous
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