MDM4 Antibody - N-terminal region (AVARP06007_P050)

Data Sheet
 
Product Number AVARP06007_P050
Product Page www.avivasysbio.com/mdm4-antibody-n-terminal-region-avarp06007-p050.html
Name MDM4 Antibody - N-terminal region (AVARP06007_P050)
Protein Size (# AA) 490 amino acids
Molecular Weight 55kDa
NCBI Gene Id 4194
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Mdm4 p53 binding protein homolog (mouse)
Alias Symbols HDMX, MDMX, MRP1, BMFS6
Peptide Sequence Synthetic peptide located within the following region: IPSQDQLKQSAEESSTSRKRTTEDDIPTLPTSEHKCIHSREDEDLIENLA
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Danovi,D., et al., (2004) Mol. Cell. Biol. 24 (13), 5835-5843
Description of Target MDM4 inhibits p53- and p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. It inhibits degradation of MDM2. It can reverse MDM2-targeted degradation of p53 while maintaining suppression of p53 transactivation and apoptotic functions. The human MDM4 gene, which plays a role in apoptosis, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal. The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein. The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested. MDM4 protein produced by in vitro translation interacts with p53 via a binding domain located in the N-terminal region of the MDM4 protein. MDM4 shows significant structural similarity to p53-binding protein MDM2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Protein Interactions RNF115; CAPN7; CD160; MDM2; TP53; UBC; MDM4; USP2; YWHAG; UBE2D2; PRKAA1; BCL2; RPS20; RPS15; RPL37; YWHAQ; HSP90AA1; CSNK1A1; PPP1CC; PPP1CB; PPP1CA; RB1; APP; UBE2D3; UBE2D1; MNAT1; ABL1; LRSAM1; TRIM55; TRIM46; MKRN3; YWHAZ; YWHAE; YWHAB; CDKN1A; RFWD2
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-MDM4 (AVARP06007_P050) antibody
Blocking Peptide For anti-MDM4 (AVARP06007_P050) antibody is Catalog # AAPS31602
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human MDM4
Uniprot ID O15151
Protein Name Protein Mdm4
Publications

Schlaeger, C. et al. Etiology-dependent molecular mechanisms in human hepatocarcinogenesis. Hepatology 47, 511-20 (2008). 18161050

Protein Accession # NP_002384
Purification Affinity Purified
Nucleotide Accession # NM_002393
Tested Species Reactivity Human
Gene Symbol MDM4
Predicted Species Reactivity Human, Cow, Dog, Horse, Pig, Rabbit
Application WB
Predicted Homology Based on Immunogen Sequence Cow: 80%; Dog: 93%; Horse: 93%; Human: 100%; Pig: 86%; Rabbit: 80%
Image 1
Human HepG2
WB Suggested Anti-MDM4 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: HepG2 cell lysate
Image 2
Human MCF-7
Lanes:
1. 10 ug MCF7 cell lysate
2. 10 ug MCF7 cell lysate
3. 10 ug MCF7 cell lysate
4. 10 ug MCF7 cell lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-Rabbit HRP
Secondary Antibody Dilution:
1:10,000
Gene Name:
MDM4
Submitted by:
Anonymous
 

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