MAPK14 Antibody - C-terminal region (AVARP06004_P050)

Data Sheet
 
Product Number AVARP06004_P050
Product Page www.avivasysbio.com/mapk14-antibody-c-terminal-region-avarp06004-p050.html
Name MAPK14 Antibody - C-terminal region (AVARP06004_P050)
Protein Size (# AA) 360 amino acids
Molecular Weight 41kDa
NCBI Gene Id 1432
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Mitogen-activated protein kinase 14
Alias Symbols RK, p38, CSBP, EXIP, Mxi2, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, SAPK2A, p38ALPHA
Peptide Sequence Synthetic peptide located within the following region: YHDPDDEPVADPYDQSFESRDLLIDEWKSLTYDEVISFVPPPLDQEEMES
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Aleman,M., et al., (2004) Infect. Immun. 72 (9), 5150-5158
Description of Target MAPK14 encodes a protein which is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response.
Protein Interactions MAPKAPK2; ATF2; PDX1; UBC; MARCKSL1; MAP2K3; MAPK14; ATF6; TCEAL1; USP11; PTPN11; OXCT1; NME1; WDR1; HSPD1; TYMS; ADSS; IQGAP1; MKNK1; MAPKAPK3; CNBP; AKT1; CCDC8; OBSL1; RRP1B; RPS6KA4; SRPK1; MAP2K6; MAPK11; NEB; MAZ; MKNK2; EEF2; DUSP9; ZFP36L1; ADRB2;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-MAPK14 (AVARP06004_P050) antibody
Blocking Peptide For anti-MAPK14 (AVARP06004_P050) antibody is Catalog # AAP30459 (Previous Catalog # AAPP01043)
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human MAPK14
Uniprot ID Q16539
Protein Name Mitogen-activated protein kinase 14
Sample Type Confirmation

MAPK14 is strongly supported by BioGPS gene expression data to be expressed in Jurkat

Protein Accession # NP_001306
Purification Affinity Purified
Nucleotide Accession # NM_001315
Tested Species Reactivity Human, Mouse
Gene Symbol MAPK14
Predicted Species Reactivity Human, Mouse, Rat, Cow, Horse, Pig, Rabbit, Sheep
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Cow: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%
Image 1
Human Jurkat
WB Suggested Anti-MAPK14 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Jurkat cell lysateMAPK14 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells
Image 2
Human Liver
Human Liver
Image 3
Human Jurkat Whole Cell
Host: Rabbit
Target Name: MAPK14
Sample Tissue: Human Jurkat Whole Cell
Antibody Dilution: 1ug/ml
Image 4
Human Bone Marrow
Rabbit Anti-MAPK14 Antibody
Catalog Number: AVARP06004_P050
Formalin Fixed Paraffin Embedded Tissue: Human Bone Marrow Tissue
Observed Staining: Cytoplasm, Nucleus
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
Image 5
Mouse Kidney
Host: Mouse
Target Name: MAPK14
Sample Tissue: Mouse Kidney
Antibody Dilution: 1ug/ml
 

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