| Product Number |
AVARP03048_P050 |
| Product Page |
www.avivasysbio.com/cdc34-antibody-middle-region-avarp03048-p050.html |
| Name |
CDC34 Antibody - middle region (AVARP03048_P050) |
| Protein Size (# AA) |
236 amino acids |
| Molecular Weight |
27kDa |
| NCBI Gene Id |
997 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Cell division cycle 34 homolog (S. cerevisiae) |
| Alias Symbols |
UBC3, UBCH3, UBE2R1, E2-CDC34 |
| Peptide Sequence |
Synthetic peptide located within the following region: TLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVEEEADSCFGDDEDDSGTEE |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Gazdoiu,S., (2007) Mol. Cell. Biol. 27 (20), 7041-7052 |
| Description of Target |
CDC34 catalyzes the covalent attachment of ubiquitin to other proteins. CDC34 may be involved in degradation of katenin.The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Protein Interactions |
FBXW11; UBC; RBX1; SIAH1; SDCBP; PPARG; ZNRF1; FBXW7; BTRC; UBA1; CDC34; HIST1H2BA; UBI4; MARCH1; MARCH2; GNPDA1; USP5; WARS; RAD23A; ANAPC11; ING4; FBXL2; Rag1; FBXO32; ITCH; NEDD4L; WWP2; TRIM28; RNF7; CUL1; CUL3; VHL; TRIM21; SKP2; CDKN1B; CCNE1; ZFAND |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-CDC34 (AVARP03048_P050) antibody |
| Blocking Peptide |
For anti-CDC34 (AVARP03048_P050) antibody is Catalog # AAP30152 (Previous Catalog # AAPP00309) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human CDC34 |
| Uniprot ID |
P49427 |
| Protein Name |
Ubiquitin-conjugating enzyme E2 R1 |
| Protein Accession # |
NP_004350 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_004359 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
CDC34 |
| Predicted Species Reactivity |
Rat, Cow, Dog |
| Application |
WB, IHC |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 92%; Rat: 92% |
| Image 1 | Human Thymus
 | WB Suggested Anti-CDC34 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: Human Thymus |
|
| Image 2 | Human heart
 | Rabbit Anti-CDC34 Antibody
Catalog Number: AVARP03048_P050
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Cytoplasmic near intercalated discs
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec |
|
