| Product Number |
AVARP03004_P050 |
| Product Page |
www.avivasysbio.com/cdk9-antibody-n-terminal-region-avarp03004-p050.html |
| Name |
CDK9 Antibody - N-terminal region (AVARP03004_P050) |
| Protein Size (# AA) |
372 amino acids |
| Molecular Weight |
43kDa |
| NCBI Gene Id |
1025 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Cyclin-dependent kinase 9 |
| Alias Symbols |
TAK, C-2k, CTK1, CDC2L4, PITALRE |
| Peptide Sequence |
Synthetic peptide located within the following region: MAKQYDSVECPFCDEVSKYEKLAKIGQGTFGEVFKARHRKTGQKVALKKV |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Sabo,A., (2008) Mol. Cell. Biol. 28 (7), 2201-2212 |
| Description of Target |
CDK9 is the member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to production elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II), SUPT5H and RDBP. The CDK9/cyclin-K complex has also a kinase activity toward CTD of RNAP II and can substitute for P-TEFb in vitro.The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Protein Interactions |
UBC; MEPCE; tat; LARP7; BRD4; CCNT1; ATM; SUMO2; AFF1; HEXIM1; FKBP5; RNF20; MSL2; RNF40; HIST2H2BE; POLR2A; MSL1; CDK9; SMC2; PLEC; CCAR2; SMC4; STIP1; MLLT3; MLLT1; CDK12; AFF4; FGFR1; CCNT2; AFF3; FBXO25; CUL1; SKIV2L; ATP1A1; SUPT5H; RPS4X; RN7SK; HEX |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-CDK9 (AVARP03004_P050) antibody |
| Blocking Peptide |
For anti-CDK9 (AVARP03004_P050) antibody is Catalog # AAP30157 (Previous Catalog # AAPP00314) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human CDK9 |
| Uniprot ID |
P50750 |
| Protein Name |
Cyclin-dependent kinase 9 |
| Protein Accession # |
NP_001252 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_001261 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
CDK9 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Pig, Zebrafish |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 92%; Dog: 100%; Human: 100%; Mouse: 92%; Pig: 92%; Rat: 92%; Zebrafish: 92% |
| Image 1 | Human 293T
 | WB Suggested Anti-CDK9 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Transfected 293T |
|
| Image 2 | Human Ovary
 | Rabbit Anti-CDK9 Antibody
Catalog Number: AVARP03004_P050
Formalin Fixed Paraffin Embedded Tissue: Human Ovary Tissue
Observed Staining: Cytoplasm
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec |
|
