| Product Number |
AVARP02058_P050 |
| Product Page |
www.avivasysbio.com/ywhae-antibody-middle-region-avarp02058-p050.html |
| Name |
YWHAE Antibody - middle region (AVARP02058_P050) |
| Protein Size (# AA) |
255 amino acids |
| Molecular Weight |
29kDa |
| NCBI Gene Id |
7531 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide |
| Alias Symbols |
MDS, HEL2, MDCR, KCIP-1, 14-3-3E |
| Peptide Sequence |
Synthetic peptide located within the following region: ELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNKEALQDVEDEN |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Tak,H., (2007) Cell. Signal. 19 (11), 2379-2387 |
| Description of Target |
YWHAE is an adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. YWHAE binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. The binding generally results in the modulation of the activity of the binding partner.This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Protein Interactions |
DTL; HUWE1; FAM53C; FBXW11; SORBS2; UBC; ABL1; GRB10; SIK2; CEP57; SIK3; TP53; IRS4; CBL; TUBB2A; TWF2; PROSC; FERMT2; PDIA4; RAP1GDS1; PTMA; PPP1R2; PDHB; PAK2; PAFAH1B2; YWHAH; XPO1; VCL; GSS; G6PD; EIF5; TBCB; CAPNS1; CAPN2; ALDH7A1; ATIC; ASNS; SCPEP1 |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-YWHAE (AVARP02058_P050) antibody |
| Blocking Peptide |
For anti-YWHAE (AVARP02058_P050) antibody is Catalog # AAP30642 (Previous Catalog # AAPP01295) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human YWHAE |
| Uniprot ID |
P62258 |
| Protein Name |
14-3-3 protein epsilon |
| Protein Accession # |
NP_006752 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_006761 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
YWHAE |
| Predicted Species Reactivity |
Mouse, Dog, Guinea Pig, Zebrafish |
| Application |
WB |
| Predicted Homology Based on Immunogen Sequence |
Dog: 100%; Guinea Pig: 100%; Mouse: 100%; Zebrafish: 100% |
| Image 1 | Human Spleen
 | WB Suggested Anti-YWHAE Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: Human Spleen |
|
| Image 2 | Human NT-2, mouse brain extracts
 | Sample Type: 1. Human NT-2 cells (60ug)
2. mouse brain extracts (80ug)
Primary Antibody Dilution: 2ug/ml
Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience
Secondary Antibody Dilution: 1: 20,000
Image Submitted by: Yuzhi Chen
University of Arkansas for Medical Science |
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