BAX Antibody - N-terminal region (AVARP02020_T100)

Data Sheet
 
Product Number AVARP02020_T100
Product Page www.avivasysbio.com/bax-antibody-n-terminal-region-avarp02020-t100.html
Name BAX Antibody - N-terminal region (AVARP02020_T100)
Protein Size (# AA) 192 amino acids
Molecular Weight 21kDa
NCBI Gene Id 581
Host Rabbit
Clonality Polyclonal
Concentration 1.0 mg/ml
Gene Full Name BCL2-associated X protein
Alias Symbols BCL2L4
Peptide Sequence Synthetic peptide located within the following region: MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPV
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Yamaguchi,H., et al., (2004) J. Biol. Chem. 279 (38), 39431-39437
Description of Target BAX encodes a protein that belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. BAX expression is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis.
Protein Interactions CYCS; ZBTB24; UBC; CASP9; CASP8; CASP7; CASP3; BCL2L1; BCL2; CLU; MDM2; MCL1; BAX; BAG3; YWHAB; VCP; CRYAB; CRYAA; BAK1; XIAP; TP53; KCNA3; HSPA4; XRCC6; XRCC5; HDAC6; BAD; PSMC5; PSMC3; PARK7; UHRF2; RNF144B; OYE2; TOMM22; TOMM40; MEPCE; MOAP1; BBC3; PPP
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-BAX (AVARP02020_T100) antibody
Blocking Peptide For anti-BAX (AVARP02020_T100) antibody is Catalog # AAP30231 (Previous Catalog # AAPP00401)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human BAX
Uniprot ID Q07812
Protein Name Apoptosis regulator BAX
Publications

De Martino, L. et al. Bid cleavage, cytochrome c release and caspase activation in canine coronavirus-induced apoptosis. Vet. Microbiol. 141, 36-45 (2010). 19781871

Honda, A. et al. Activation of caspase 3, 9, 12, and Bax in masseter muscle of mdx mice during necrosis. J. Muscle Res. Cell Motil. 28, 243-7 (2007). 17952618

Protein Accession # NP_620116
Purification Protein A purified
Nucleotide Accession # NM_138761
Tested Species Reactivity Human
Gene Symbol BAX
Predicted Species Reactivity Human, Cow, Dog, Pig
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Human: 100%; Pig: 100%
Image 1
Human NT-2 and Mouse WT brain
Lanes:
1. Human NT-2 cells (60ug) lysate 2. Mouse WT brain extract (80ug)
Primary Antibody Dilution:
2ug/ml
Secondary Antibody:
IRDye 800CW goat anti-rabbit from Li-COR Bioscience
Secondary Antibody Dilution:
1: 20,000
Gene Name:
BAX
Submitted by:
Dr. Yuzhi Chen, University of Arkansas for Medical Science
Image 2
Human HepG2
WB Suggested Anti-BAX Antibody Titration: 1.25ug/ml
Positive Control: HepG2 cell lysate
Image 3
Human kidney
Human kidney
Image 4
Human Heart
Rabbit Anti-BAX Antibody
Catalog Number: AVARP02020
Paraffin Embedded Tissue: Human cardiac cell
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X
 

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