Product Number |
AVARP02020_T100 |
Product Page |
www.avivasysbio.com/bax-antibody-n-terminal-region-avarp02020-t100.html |
Name |
BAX Antibody - N-terminal region (AVARP02020_T100) |
Protein Size (# AA) |
192 amino acids |
Molecular Weight |
21kDa |
NCBI Gene Id |
581 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
1.0 mg/ml |
Gene Full Name |
BCL2-associated X protein |
Alias Symbols |
BCL2L4 |
Peptide Sequence |
Synthetic peptide located within the following region: MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPV |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Yamaguchi,H., et al., (2004) J. Biol. Chem. 279 (38), 39431-39437 |
Description of Target |
BAX encodes a protein that belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. BAX expression is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. |
Protein Interactions |
CYCS; ZBTB24; UBC; CASP9; CASP8; CASP7; CASP3; BCL2L1; BCL2; CLU; MDM2; MCL1; BAX; BAG3; YWHAB; VCP; CRYAB; CRYAA; BAK1; XIAP; TP53; KCNA3; HSPA4; XRCC6; XRCC5; HDAC6; BAD; PSMC5; PSMC3; PARK7; UHRF2; RNF144B; OYE2; TOMM22; TOMM40; MEPCE; MOAP1; BBC3; PPP |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-BAX (AVARP02020_T100) antibody |
Blocking Peptide |
For anti-BAX (AVARP02020_T100) antibody is Catalog # AAP30231 (Previous Catalog # AAPP00401) |
Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human BAX |
Uniprot ID |
Q07812 |
Protein Name |
Apoptosis regulator BAX |
Publications |
De Martino, L. et al. Bid cleavage, cytochrome c release and caspase activation in canine coronavirus-induced apoptosis. Vet. Microbiol. 141, 36-45 (2010). 19781871
Honda, A. et al. Activation of caspase 3, 9, 12, and Bax in masseter muscle of mdx mice during necrosis. J. Muscle Res. Cell Motil. 28, 243-7 (2007). 17952618 |
Protein Accession # |
NP_620116 |
Purification |
Protein A purified |
Nucleotide Accession # |
NM_138761 |
Tested Species Reactivity |
Human |
Gene Symbol |
BAX |
Predicted Species Reactivity |
Human, Cow, Dog, Pig |
Application |
IHC, WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Human: 100%; Pig: 100% |
Image 1 | Human NT-2 and Mouse WT brain
| Lanes: 1. Human NT-2 cells (60ug) lysate 2. Mouse WT brain extract (80ug) Primary Antibody Dilution: 2ug/ml Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience Secondary Antibody Dilution: 1: 20,000 Gene Name: BAX Submitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science
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Image 2 | Human HepG2
| WB Suggested Anti-BAX Antibody Titration: 1.25ug/ml Positive Control: HepG2 cell lysate |
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Image 3 | Human kidney
| Human kidney |
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Image 4 | Human Heart
| Rabbit Anti-BAX Antibody Catalog Number: AVARP02020 Paraffin Embedded Tissue: Human cardiac cell Cellular Data: Epithelial cells of renal tubule Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X |
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