TRADD Antibody - middle region (AVARP02012_P050)

• Product Number: AVARP02012_P050
• Name: TRADD Antibody - middle region (AVARP02012_P050)
• Protein Size (# AA): 312 amino acids
• Molecular Weight: 34kDa
• NCBI Gene Id: 8717
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: TNFRSF1A-associated via death domain
• Alias Symbols: Hs.89862
• Peptide Sequence: Synthetic peptide located within the following region: YEQAFQLLRRFVQAEGRRATLQRLVEALEENELTSLAEDLLGLTDPNGGL
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Miyagawa,H., (2008) Rheumatology (Oxford) 47 (2), 158-164
• Description of Target: TRADD is the adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B.The protein encoded by this gene is a death domain containing adaptor molecule that interacts with TNFRSF1A/TNFR1 and mediates programmed cell death signaling and NF-kappaB activation. This protein binds adaptor protein TRAF2, reduces the recruitment of inhibitor-of-apoptosis proteins (IAPs) by TRAF2, and thus suppresses TRAF2 mediated apoptosis. This protein can also interact with receptor TNFRSF6/FAS and adaptor protein FADD/MORT1, and is involved in the Fas-induced cell death pathway. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Protein Interactions: FADD; UBC; TNF; RIPK1; TNFRSF1A; ZMYND11; TOMM70A; PDLIM7; LRRK2; OPTN; TRADD; TRAF2; TRAF1; TNFRSF1B; GFRA1; CHUK; FAS; DAB2IP; EDAR; CDKN2A; TRIP12; LMP1; CASP8; SH3KBP1; USP2; TNFRSF25; PPP2R1A; TRPC4AP; HIPK2; TNFRSF21; CNTRL; TNFRSF10B; TNFRSF10A; BC
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-TRADD (AVARP02012_P050) antibody is Catalog # AAP30256 (Previous Catalog # AAPP00426)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TRADD
• Uniprot ID: Q15628
• Protein Name: Tumor necrosis factor receptor type 1-associated DEATH domain protein
• Sample Type Confirmation:

  There is BioGPS gene expression data showing that TRADD is expressed in DU145, HEK293T

• Protein Accession #: NP_003780
• Purification: Affinity Purified
• Nucleotide Accession #: NM_003789
• Tested Species Reactivity: Human
• Gene Symbol: TRADD
• Predicted Species Reactivity: Human, Dog, Horse
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Dog: 76%; Horse: 76%; Human: 100%
• Image 1: Human DU145
  
  WB Suggested Anti-TRADD Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:312500
  Positive Control: DU145 cell lysateThere is BioGPS gene expression data showing that TRADD is expressed in DU145
• Image 2: Human 293T
  
  Lanes:
  Lane 1: 10ug Tradd-HA-Strep-stable expression 293TREXFlpIn cells-Doxycycline induced
  Lane 2: 10ug ITradd-HA-Strep-stable expression 293TREXFlpIn cells-non-induced
  Lane 3: 10ug siRNA scrambled-MDA-MB-231 cells
  Lane 4: siRNA Tradd-MDA-MB-231 cells
  Primary Antibody Dilution:
  1:1000
  Secondary Antibody:
  Anti-rabbit HRP
  Secondary Antibody Dilution:
  1:2000
  Gene Name:
  TRADD
  Submitted by:
  Dr. Tencho Tenev, The Breakthrough Breast Cancer Research Centre, Institute of Cancer Research
  There is BioGPS gene expression data showing that TRADD is expressed in HEK293T