CDC42 Antibody - N-terminal region (AVARP01017_P050)

• Product Number: AVARP01017_P050
• Name: CDC42 Antibody - N-terminal region (AVARP01017_P050)
• Protein Size (# AA): 191 amino acids
• Molecular Weight: 21kDa
• NCBI Gene Id: 998
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Cell division cycle 42 (GTP binding protein, 25kDa)
• Alias Symbols: TKS, G25K, CDC42Hs
• Peptide Sequence: Synthetic peptide located within the following region: DNYAVTVMIGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSP
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Black,S.A. (2008) J. Biol. Chem. 283 (16), 10835-10847
• Description of Target: CDC42 is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants.The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants.
• Protein Interactions: PARD6B; CDC42EP1; CDC42EP2; PAK2; UBC; TRAF2; PAK1; ARHGDIA; BIRC2; TNK2; TCEB2; EPRS; LARS; RUVBL1; NEK6; WIPF1; WASL; RIOK3; SSX2IP; DIAPH3; BAIAP2; PAK3; ZNF420; IQGAP1; RAP1A; WAS; TGFBR2; TGFBR1; ARHGEF6; LRRK2; FGD3; ARHGAP1; SH3RF3; BCAR1; USP6; EL
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-CDC42 (AVARP01017_P050) antibody is Catalog # AAP30406 (Previous Catalog # AAPP00929)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CDC42
• Uniprot ID: P60953
• Protein Name: Cell division control protein 42 homolog
• Sample Type Confirmation:

  CDC42 is supported by BioGPS gene expression data to be expressed in 721_B

• Protein Accession #: NP_001782
• Purification: Affinity Purified
• Nucleotide Accession #: NM_001791
• Tested Species Reactivity: Human
• Gene Symbol: CDC42
• Predicted Species Reactivity: Dog, Rabbit
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Dog: 100%; Rabbit: 100%
• Image 1: Human 721_B
  
  WB Suggested Anti-CDC42 Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:62500
  Positive Control: 721_B cell lysateCDC42 is supported by BioGPS gene expression data to be expressed in 721_B
• Image 2: Human Bone Marrow
  
  Rabbit Anti-CDC42 Antibody
  Catalog Number: AVARP01017_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Bone Marrow Tissue
  Observed Staining: Cytoplasm, Plasma membrane
  Primary Antibody Concentration: 1:100
  Other Working Concentrations: 1:600
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 - 2.0 sec
• Image 3: Human liver tissue
  
  Rabbit Anti-CDC42 Antibody
  Catalog Number: AVARP01017_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue
  Observed Staining: Cytoplasm in hepatocytes
  Primary Antibody Concentration: 1:100
  Other Working Concentrations: N/A
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 - 2.0 sec