| Product Number |
AVARP00019_P050 |
| Product Page |
www.avivasysbio.com/nlrc4-antibody-c-terminal-region-avarp00019-p050.html |
| Name |
NLRC4 Antibody - C-terminal region (AVARP00019_P050) |
| Protein Size (# AA) |
1024 amino acids |
| Molecular Weight |
116kDa |
| NCBI Gene Id |
58484 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
NLR family, CARD domain containing 4 |
| Description |
|
| Alias Symbols |
CLAN, IPAF, AIFEC, CLAN1, CLANA, CLANB, CLANC, CLAND, FCAS4, CARD12, CLR2.1 |
| Peptide Sequence |
Synthetic peptide located within the following region: QLNLAGNRVSSDGWLAFMGVFENLKQLVFFDFSTKEFLPDPALVRKLSQV |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Clark,H.F., et al., (2003) Genome Res. 13 (10), 2265-2270 |
| Description of Target |
In C. elegans, Ced4 binds and activates Ced3, an apoptotic initiator caspase, via caspase-associated recruitment domains (CARDs). Human Ced4 homologs include APAF1, NOD1, and NOD2. These proteins have at least 1 N-terminal CARD domain followed by a centrally located nucleotide-binding domain (NBD or NACHT) and a C-terminal regulatory domain, found only in mammals, that contains either WD40 repeats or leucine-rich repeats (LRRs). CARD12 is a member of the Ced4 family and can induce apoptosis. |
| Protein Interactions |
UBC; XK; ALB; NLRC4; PSMC5; CASP8; NLRP4; PYCARD; NOD2; BCL10; CASP1; NLRP1; NLRP3; NOD1; NAIP; |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-NLRC4 (AVARP00019_P050) antibody |
| Specificity |
This antibody will recognize NLRC4 isoform 1 (116kD) and isoform 2 (40kD). |
| Blocking Peptide |
For anti-NLRC4 (AVARP00019_P050) antibody is Catalog # AAP30490 (Previous Catalog # AAPP01126) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human NLRC4 |
| Uniprot ID |
Q9NPP4 |
| Protein Name |
NLR family CARD domain-containing protein 4 |
| Publications |
Inflammasomes are important mediators of cyclophosphamide-induced bladder inflammation. Am J Physiol Renal Physiol. 306, F299-308 (2014). 24285499
The potential repertoire of the innate immune system in the bladder: expression of pattern recognition receptors in the rat bladder and a rat urothelial cell line (MYP3 cells). Int Urol Nephrol. 47, 1953-64 (2015). 26490556 |
| Protein Accession # |
NP_067032 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_021209 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
NLRC4 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Guinea Pig |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Guinea Pig: 80%; Human: 100%; Mouse: 86%; Rat: 80% |
| Image 1 | Human Smooth Muscle
 | Human Smooth Muscle |
|
| Image 2 | Human HepG2
 | WB Suggested Anti-NLRC4 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: HepG2 cell lysate |
|
