ATP2B1 Polyclonal Antibody (Human) (ATP00033_T100)

• Name: ATP2B1 Polyclonal Antibody (Human) (ATP00033_T100)
• Protein Size (# AA): 1220 amino acids
• Molecular Weight: 135 kDa
• Isotype: IgG
• NCBI Gene Id: 490
• Clonality: Polyclonal
• Concentration: 1 mg/ml
• Gene Full Name: ATPase plasma membrane Ca2+ transporting 1
• Alias Symbols: MRD66; PMCA1; PMCA1kb
• Product Format: PBS w/ 0.09% sodium azide
• Description of Target: The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 1. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: Human 6XHis-MBP-(683-833)
• Uniprot ID: P20020
• Protein Name: Plasma membrane calcium-transporting ATPase 1
• Purification: Protein A
• Gene Symbol: ATP2B1
• Predicted Species Reactivity: Human
• Application: WB
• Image 1: Immunogen: Recombinant protein representing aa 683-833 of human ATP2B1 (green) depicted within in the full length structure prediction (blue) via AlphaFold. (https://alphafold.ebi.ac.uk/).
  
  Immunogen: Recombinant protein representing aa 683-833 of human ATP2B1 (green) depicted within in the full length structure prediction (blue) via AlphaFold. (https://alphafold.ebi.ac.uk/).
• Image 2: 20 ug of the indicated human whole cell extract was loaded onto a 4-20% SDS-PAGE gel. After electrophoresis, proteins were transferred to a PVDF membrane and probed with 3 ug/mL of antibody. Secondary detection was performed with 0.08 ug/mL of HRP-conjuga
  
  20 ug of the indicated human whole cell extract was loaded onto a 4-20% SDS-PAGE gel. After electrophoresis, proteins were transferred to a PVDF membrane and probed with 3 ug/mL of antibody. Secondary detection was performed with 0.08 ug/mL of HRP-conjugated donkey anti-rabbit IgG antibody. Images were captured after development of membrane following exposure to AvivaBlot™ Femto ECL Reagent (OKCF00002).