| Product Number |
ARP87308_P050 |
| Product Page |
www.avivasysbio.com/itch-antibody-middle-region-arp87308-p050.html |
| Name |
ITCH Antibody - middle region (ARP87308_P050) |
| Protein Size (# AA) |
752 amino acids |
| Molecular Weight |
103 kDa |
| NCBI Gene Id |
83737 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
itchy E3 ubiquitin protein ligase |
| Alias Symbols |
AIF4, AIP4, ADMFD, NAPP1 |
| Peptide Sequence |
Synthetic peptide located within the following region: KPSRPPRPSRPPPPTPRRPASVNGSPSATSESDGSSTGSLPPTNTNTNTS |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Description of Target |
This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-ITCH (ARP87308_P050) antibody |
| Blocking Peptide |
For anti-ITCH (ARP87308_P050) antibody is Catalog # AAP87308 |
| Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human ITCH |
| Uniprot ID |
Q96J02-3 |
| Protein Name |
E3 ubiquitin-protein ligase Itchy homolog |
| Protein Accession # |
NP_001244066.1 |
| Purification |
Affinity purified |
| Nucleotide Accession # |
NM_001257137.2 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
ITCH |
| Predicted Species Reactivity |
Human |
| Application |
WB |
| Image 1 | Western Blot
 | 25 ug of the indicated Human whole cell extracts was loaded onto a 6-18% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. The canonical isoform is 103 kDa, while a ~99 kDa isoform is observed in these samples. |
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