Product Number |
ARP74061_P050 |
Product Page |
www.avivasysbio.com/melk-antibody-middle-region-arp74061-p050.html |
Name |
MELK Antibody - middle region (ARP74061_P050) |
Protein Size (# AA) |
457 amino acids |
Molecular Weight |
50kDa |
NCBI Gene Id |
9833 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Alias Symbols |
HPK38 |
Peptide Sequence |
Synthetic peptide located within the following region: MEETPKRKGAKVFGSLERGLDKVITVLTRSKRKGSARDGPRRLKLHYNVT |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
N/A |
Description of Target |
MELK is a serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. It acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. And it plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. It may also play a role in primitive hematopoiesis. |
Protein Interactions |
UBC; YGR054W; Acaca; CSN1S1; MELK; MBP; LMNB1; BABAM1; ZNF622; SF3B1; EIF2S1; CDC5L; PPP1R8; HIST1H1B; CDC25B; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-MELK (ARP74061_P050) antibody |
Blocking Peptide |
For anti-MELK (ARP74061_P050) antibody is Catalog # AAP74061 |
Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of Human MELK |
Uniprot ID |
Q14680-3 |
Purification |
Affinity purified |
Tested Species Reactivity |
Human |
Gene Symbol |
MELK |
Predicted Species Reactivity |
Human |
Application |
WB |
Image 1 | Human A549 Whole Cell
| Host: Rabbit Target Name: MELK Sample Tissue: Human A549 Whole Cell Antibody Dilution: 1ug/ml |
|
|