FLNC Antibody - N-terminal region (ARP64454_P050)

Data Sheet
 
Product Number ARP64454_P050
Product Page www.avivasysbio.com/flnc-antibody-n-terminal-region-arp64454-p050.html
Name FLNC Antibody - N-terminal region (ARP64454_P050)
Protein Size (# AA) 2692 amino acids
Molecular Weight 296kDa
NCBI Gene Id 2318
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Filamin C, gamma
Alias Symbols ABPA, ABPL, FLN2, MFM5, MPD4, RCM5, CMH26, ABP-280, ABP280A
Peptide Sequence Synthetic peptide located within the following region: VGKSADFVVEAIGTEVGTLGFSIEGPSQAKIECDDKGDGSCDVRYWPTEP
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Description of Target This gene encodes one of three related filamin genes, specifically gamma filamin. These filamin proteins crosslink actin filaments into orthogonal networks in cortical cytoplasm and participate in the anchoring of membrane proteins for the actin cytoskeleton. Three functional domains exist in filamin: an N-terminal filamentous actin-binding domain, a C-terminal self-association domain, and a membrane glycoprotein-binding domain. Two transcript variants encoding different isoforms have been found for this gene.
Protein Interactions RAPGEF2; TRIM63; TRIM55; XIRP1; XIRP2; SYNPO2; UBC; KCMF1; CTTNBP2NL; ANKRD28; ARFGEF2; NCAPD2; TXNL1; PSMD13; PSMD12; PSMD3; NUP88; IKBKB; FLNB; AP3S1; NPM1; IGSF8; FN1; IQCB1; TP63; VCAM1; RPS6KA2; DYNLRB1; NUP133; SAP30BP; SF3A3; SRSF9; ENO1; EEF1G; EE
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-FLNC (ARP64454_P050) antibody
Blocking Peptide For anti-FLNC (ARP64454_P050) antibody is Catalog # AAP64454
Immunogen The immunogen is a synthetic peptide directed towards the N-terminal region of Human FLNC
Uniprot ID Q14315
Protein Name Filamin-C
Publications

FLNC Gene Splice Mutations Cause Dilated Cardiomyopathy. JACC Basic Transl Sci. 1, 344-359 (2016). 28008423

Protein Accession # NP_001120959
Purification Affinity Purified
Nucleotide Accession # NM_001127487
Tested Species Reactivity Human
Gene Symbol FLNC
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
Application WB
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
Image 1
Human HepG2
WB Suggested Anti-FLNC Antibody
Titration: 1.0 ug/ml
Positive Control: HepG2 Whole Cell
Image 2

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
 

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