CLU Antibody - C-terminal region (ARP61142_P050)

Data Sheet
 
Product Number ARP61142_P050
Product Page www.avivasysbio.com/clu-antibody-c-terminal-region-arp61142-p050.html
Name CLU Antibody - C-terminal region (ARP61142_P050)
Protein Size (# AA) 501 amino acids
Molecular Weight 58kDa
NCBI Gene Id 1191
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Clusterin
Description
Alias Symbols CLI, AAG4, APOJ, CLU1, CLU2, KUB1, SGP2, APO-J, SGP-2, SP-40, TRPM2, TRPM-2, NA1/NA2
Peptide Sequence Synthetic peptide located within the following region: CREILSVDCSTNNPSQAKLRRELDESLQVAERLTRKYNELLKSYQWKMLN
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Description of Target The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.
Protein Interactions HYOU1; HSP90B1; HSPA5; BAX; TUFM; NUCB2; HSPA1L; TUBB6; MDM2; FOS; CDK19; APOA1; KEAP1; POLR1C; RPL23; KLF11; BAG6; RBBP8; MAPK9; PDIA3; CYP2E1; BCL2L1; TSC22D4; MDC1; LIG4; PAXIP1; CLUAP1; IL24; PRNP; PON1; HSPD1; APP; FBXO6; LYZ; LRP2; HBA1; XRCC6; ELN;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Enhanced Validation
Relative Expression (Western Blot) Avivasheild
Datasheets/Manuals Printable datasheet for anti-CLU (ARP61142_P050) antibody
Blocking Peptide For anti-CLU (ARP61142_P050) antibody is Catalog # AAP61142 (Previous Catalog # AAPP47252)
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human CLU
Uniprot ID P10909
Protein Name Clusterin
Publications

Clusterin secretion is attenuated by the proinflammatory cytokines interleukin-1β and tumor necrosis factor-α in models of cartilage degradation. J Orthop Res. (2020) 32725904

Protein Accession # NP_001822
Purification Affinity Purified
Nucleotide Accession # NM_001831
Tested Species Reactivity Human, Horse
Gene Symbol CLU
Predicted Species Reactivity Human, Mouse, Rat, Guinea Pig, Horse, Rabbit
Application WB, IHC
Predicted Homology Based on Immunogen Sequence Guinea Pig: 86%; Horse: 100%; Human: 100%; Mouse: 86%; Rabbit: 91%; Rat: 86%
Image 1
Human Fetal Brain
CLU antibody - C-terminal region (ARP61142_P050) validated by WB using Fetal Brain Lysate at 1ug/ml.
Image 2
Human HK2
Lanes:
1. 40 ug human HK2 cell (kidney proximal tubular cell line) lysate
2. 40 ug H2O2 treated human HK2 Cell lysate
3. 40 ug H2O2 treated human HK2 Cell lysate
4. 40 ug H2O2 treated human HK2 Cell lysate
5. 40 ug H2O2 treated human HK2 Cell lysate
6. 40 ug H2O2 treated human HK2 Cell lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Goat anti-Rabbit HRP
Secondary Antibody Dilution:
1:2000
Gene Name:
CLU
Submitted by:
The University of Queensland, School of Medicine, Centre for Kidney Disease Research
Image 3
Human heart
Rabbit Anti-CLU Antibody
Catalog Number: ARP61142_P050
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Cytoplasmic
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
Image 4
Human liver tissue
Rabbit Anti-CLU Antibody
Catalog Number: ARP61142_P050
Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue
Observed Staining: Cytoplasm in hepatocytes
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
 

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