EGFR Antibody - middle region (ARP61003_P050)

• Product Number: ARP61003_P050
• Name: EGFR Antibody - middle region (ARP61003_P050)
• Protein Size (# AA): 628 amino acids
• Molecular Weight: 69kDa
• NCBI Gene Id: 1956
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Epidermal growth factor receptor
• Alias Symbols: ERBB, ERRP, HER1, mENA, ERBB1, PIG61, NISBD2
• Peptide Sequence: Synthetic peptide located within the following region: VTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLS
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Description of Target: The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.
• Protein Interactions: SEC61A1; UBC; CBL; ERBB4; EMD; SEC61B; DCBLD2; PML; DERL1; SOCS5; EPHA2; UBASH3B; PTPRF; EIF2AK2; MAP2K3; MAP2K2; PRKAG1; PRKAA1; PPP2R5C; PLK1; PLCG1; PFKP; PFKM; PFKL; PFKFB3; PDK1; VAV2; UCK2; UBE2G2; DNM2; CTNND1; CSNK2A1; CSNK1A1; CSK; AP3S1; AP2S1;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-EGFR (ARP61003_P050) antibody is Catalog # AAP61003
• Uniprot ID: P00533
• Protein Name: Epidermal growth factor receptor
• Sample Type Confirmation:

  EGFR is strongly supported by BioGPS gene expression data to be expressed in MDA-MB231

• Protein Accession #: NP_958439
• Purification: Affinity Purified
• Nucleotide Accession #: NM_201282
• Tested Species Reactivity: Human
• Gene Symbol: EGFR
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 92%; Dog: 93%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 85%; Rabbit: 93%; Rat: 100%; Zebrafish: 75%
• Image 1: Human MDA-MB231
  
  Lanes:
  Lane 1: 4ug DRM fraction from MDA-MB-231
  Primary Antibody Dilution:
  1:1000
  Secondary Antibody:
  Donkey anti-rabbit IgG-HRP
  Secondary Antibody Dilution:
  1:10000
  Gene Name:
  EGFR
  Submitted by:
  Katarzyna Augoff, University of Wroclaw
  EGFR is strongly supported by BioGPS gene expression data to be expressed in Human MDA-MB231 cells
• Image 2: Human Thyroid Gland
  
  Rabbit Anti-EGFR Antibody
  Catalog Number: ARP61003_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Thyroid Gland Tissue
  Observed Staining: Cytoplasm
  Primary Antibody Concentration: 1:100
  Other Working Concentrations: N/A
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 - 2.0 sec
• Image 3: Human Placenta
  
  WB Suggested Anti-EGFR Antibody
  Titration: 1.0 ug/ml
  Positive Control: Placenta