COL1A1 Antibody - C-terminal region (ARP59999_P050)

Data Sheet
 
Product Number ARP59999_P050
Product Page www.avivasysbio.com/col1a1-antibody-c-terminal-region-arp59999-p050.html
Name COL1A1 Antibody - C-terminal region (ARP59999_P050)
Protein Size (# AA) 1464 amino acids
Molecular Weight 139 kDa
NCBI Gene Id 1277
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Collagen, type I, alpha 1
Alias Symbols OI1, OI2, OI3, OI4, EDSC, CAFYD, EDSARTH1
Peptide Sequence Synthetic peptide located within the following region: PPGPPSAGFDFSFLPQPPQEKAHDGGRYYRADDANVVRDRDLEVDTTLKS
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Description of Target This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.
Protein Interactions PDGFB; PDGFA; COL1A2; COL1A1; CAPN1; UBC; BRCA1; BARD1; C12orf57; RNH1; ATP13A2; ELAVL1; ACHE; TMPRSS6; P4HB; PAK1; HTRA1; MATN2; PRELP; TGFBI; BGN; VWF; ITGA2; DDR2; THBS1; TXN; SPARC; CD36; FGF7; IGFBP3; ITGA5; FN1; NID1; DCN; CD93; MMP9; MMP2; COL7A1;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Enhanced Validation
Relative Expression (Western Blot) Avivasheild
SPR Affinity Characterization Avivasheild
Datasheets/Manuals Printable datasheet for anti-COL1A1 (ARP59999_P050) antibody
Blocking Peptide For anti-COL1A1 (ARP59999_P050) antibody is Catalog # AAP59999 (Previous Catalog # AAPP46153)
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human COL1A1
Uniprot ID P02452
Protein Name Collagen alpha-1(I) chain
Protein Accession # NP_000079
Purification Affinity Purified
Nucleotide Accession # NM_000088
Tested Species Reactivity Human, Mouse
Gene Symbol COL1A1
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Guinea Pig: 86%; Horse: 93%; Human: 100%; Mouse: 86%; Rabbit: 92%; Rat: 86%
Image 1
Mouse blastocyte
Researcher: Ivan Bedzhov, PhD, Gurdon Institute, University of Cambridge
Application: IHC
Species+tissue/cell type:
Species+tissue/cell type: Mouse blastocyte
Primary antibody dilution: 1:200
Secondary antibody: Anti-rabbit-Alexa 488
Secondary antibody dilution:1:200
Image 2
Western Blot
25 ug of the indicated Human whole cell extracts was loaded onto a 6-18% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. An isoform containing the peptide sequence is present at ~ 31 kDa.
Image 3

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
 

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