Product Number |
ARP59999_P050 |
Product Page |
www.avivasysbio.com/col1a1-antibody-c-terminal-region-arp59999-p050.html |
Name |
COL1A1 Antibody - C-terminal region (ARP59999_P050) |
Protein Size (# AA) |
1464 amino acids |
Molecular Weight |
139 kDa |
NCBI Gene Id |
1277 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Collagen, type I, alpha 1 |
Alias Symbols |
OI1, OI2, OI3, OI4, EDSC, CAFYD, EDSARTH1 |
Peptide Sequence |
Synthetic peptide located within the following region: PPGPPSAGFDFSFLPQPPQEKAHDGGRYYRADDANVVRDRDLEVDTTLKS |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Description of Target |
This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. |
Protein Interactions |
PDGFB; PDGFA; COL1A2; COL1A1; CAPN1; UBC; BRCA1; BARD1; C12orf57; RNH1; ATP13A2; ELAVL1; ACHE; TMPRSS6; P4HB; PAK1; HTRA1; MATN2; PRELP; TGFBI; BGN; VWF; ITGA2; DDR2; THBS1; TXN; SPARC; CD36; FGF7; IGFBP3; ITGA5; FN1; NID1; DCN; CD93; MMP9; MMP2; COL7A1; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Enhanced Validation |
Relative Expression (Western Blot) |
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SPR Affinity Characterization |
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Datasheets/Manuals |
Printable datasheet for anti-COL1A1 (ARP59999_P050) antibody |
Blocking Peptide |
For anti-COL1A1 (ARP59999_P050) antibody is Catalog # AAP59999 (Previous Catalog # AAPP46153) |
Immunogen |
The immunogen is a synthetic peptide directed towards the C terminal region of human COL1A1 |
Uniprot ID |
P02452 |
Protein Name |
Collagen alpha-1(I) chain |
Protein Accession # |
NP_000079 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_000088 |
Tested Species Reactivity |
Human, Mouse |
Gene Symbol |
COL1A1 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit |
Application |
IHC, WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 86%; Horse: 93%; Human: 100%; Mouse: 86%; Rabbit: 92%; Rat: 86% |
Image 1 | Mouse blastocyte
| Researcher: Ivan Bedzhov, PhD, Gurdon Institute, University of Cambridge Application: IHC Species+tissue/cell type: Species+tissue/cell type: Mouse blastocyte Primary antibody dilution: 1:200 Secondary antibody: Anti-rabbit-Alexa 488 Secondary antibody dilution:1:200 |
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Image 2 | Western Blot
| 25 ug of the indicated Human whole cell extracts was loaded onto a 6-18% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. An isoform containing the peptide sequence is present at ~ 31 kDa. |
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Image 3 |
| Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.
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