| Product Number |
ARP58983_P050 |
| Product Page |
www.avivasysbio.com/casp1-antibody-middle-region-arp58983-p050.html |
| Name |
CASP1 Antibody - middle region (ARP58983_P050) |
| Protein Size (# AA) |
383 amino acids |
| Molecular Weight |
10kDa |
| NCBI Gene Id |
834 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Caspase 1, apoptosis-related cysteine peptidase |
| Description |
|
| Alias Symbols |
ICE, P45, IL1BC |
| Peptide Sequence |
Synthetic peptide located within the following region: LQTRVLNKEEMEKVKRENATVMDKTRALIDSVIPKGAQACQICITYICEE |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Description of Target |
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms. |
| Protein Interactions |
ced-3; ced-4; VAC14; BCL2L1; UBC; XK; PLA2G4B; IL33; IL1B; PARP1; BIRC3; TRAF2; CEBPB; TIRAP; CARD17; CARD16; CARD8; PLA2G4A; CDK11B; NLRP1; ATN1; ATXN3; PYCARD; NLRC4; NOD1; CASP14; RIPK2; PAK1; PARK2; NEDD4; BID; IL18; PSEN2; NFE2L2; AR; BCAP31; MAPT; L |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-CASP1 (ARP58983_P050) antibody |
| Blocking Peptide |
For anti-CASP1 (ARP58983_P050) antibody is Catalog # AAP58983 (Previous Catalog # AAPP44950) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human CASP1 |
| Uniprot ID |
P29466 |
| Protein Name |
Caspase-1 |
| Sample Type Confirmation |
CASP1 is strongly supported by BioGPS gene expression data to be expressed in 721_B |
| Protein Accession # |
NP_001214 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_001223 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
CASP1 |
| Predicted Species Reactivity |
Human, Horse, Rabbit, Yeast |
| Application |
WB, IHC |
| Predicted Homology Based on Immunogen Sequence |
Horse: 79%; Human: 100%; Rabbit: 79%; Yeast: 100% |
| Image 1 | Human 721_B
 | WB Suggested Anti-CASP1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: 721_B cell lysateCASP1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells |
|
| Image 2 | Human Lymph Node
 | Rabbit Anti-CASP1 Antibody
Catalog Number: ARP58983_P050
Formalin Fixed Paraffin Embedded Tissue: Human Lymph Node Tissue
Observed Staining: Cytoplasm
Primary Antibody Concentration: 1:600
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec |
|
