CALR Antibody - middle region (ARP58880_P050)

Data Sheet
 
Product Number ARP58880_P050
Product Page www.avivasysbio.com/calr-antibody-middle-region-arp58880-p050.html
Name CALR Antibody - middle region (ARP58880_P050)
Protein Size (# AA) 417 amino acids
Molecular Weight 46kDa
NCBI Gene Id 811
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Calreticulin
Alias Symbols RO, CRT, SSA, cC1qR, HEL-S-99n
Peptide Sequence Synthetic peptide located within the following region: ASKPEDWDERAKIDDPTDSKPEDWDKPEHIPDPDAKKPEDWDEEMDGEWE
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Description of Target Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium.
Protein Interactions HUWE1; UBC; LGR4; MDM2; RNF2; SUZ12; rev; AARSD1; THG1L; NSUN2; SH3GLB1; EEF2K; ARPC1A; ANP32B; ACTR2; ARPC1B; ARPC3; ARPC5; TPD52L2; SH3GL1; RPA3; RPA1; PRKACA; PFDN5; LPP; PARK2; UPF2; UBD; SOX2; PDIA3; CLN5; ITGA3; HLA-E; HLA-A; CALR; SLC27A2; env; MTN
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-CALR (ARP58880_P050) antibody
Blocking Peptide For anti-CALR (ARP58880_P050) antibody is Catalog # AAP58880 (Previous Catalog # AAPP44827)
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human CALR
Uniprot ID P27797
Protein Name Calreticulin
Protein Accession # NP_004334
Purification Affinity Purified
Nucleotide Accession # NM_004343
Tested Species Reactivity Human, Mouse
Gene Symbol CALR
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish
Application WB, IHC
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86%
Image 1
Human Brain
WB Suggested Anti-CALR Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human brain
Image 2
Mouse Small Intestine
Host: Mouse
Target Name: CALR
Sample Tissue: Mouse Small Intestine
Antibody Dilution: 1ug/ml
Image 3
Thyroid
Rabbit Anti-CALR antibody
Catalog Number: ARP58880
Formalin Fixed Paraffin Embedded Tissue: Human Adult Thyroid
Observed Staining: Cytoplasm in hepatocytes
Primary Antibody Concentration: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 – 2.0 sec
Image 4
Human heart
Rabbit Anti-CALR Antibody
Catalog Number: ARP58880_P050
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Cytoplasmic, plasma membrane in intercalated disc
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
 

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