Product Number |
ARP58880_P050 |
Product Page |
www.avivasysbio.com/calr-antibody-middle-region-arp58880-p050.html |
Name |
CALR Antibody - middle region (ARP58880_P050) |
Protein Size (# AA) |
417 amino acids |
Molecular Weight |
46kDa |
NCBI Gene Id |
811 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Calreticulin |
Alias Symbols |
RO, CRT, SSA, cC1qR, HEL-S-99n |
Peptide Sequence |
Synthetic peptide located within the following region: ASKPEDWDERAKIDDPTDSKPEDWDKPEHIPDPDAKKPEDWDEEMDGEWE |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Description of Target |
Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. |
Protein Interactions |
HUWE1; UBC; LGR4; MDM2; RNF2; SUZ12; rev; AARSD1; THG1L; NSUN2; SH3GLB1; EEF2K; ARPC1A; ANP32B; ACTR2; ARPC1B; ARPC3; ARPC5; TPD52L2; SH3GL1; RPA3; RPA1; PRKACA; PFDN5; LPP; PARK2; UPF2; UBD; SOX2; PDIA3; CLN5; ITGA3; HLA-E; HLA-A; CALR; SLC27A2; env; MTN |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-CALR (ARP58880_P050) antibody |
Blocking Peptide |
For anti-CALR (ARP58880_P050) antibody is Catalog # AAP58880 (Previous Catalog # AAPP44827) |
Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human CALR |
Uniprot ID |
P27797 |
Protein Name |
Calreticulin |
Protein Accession # |
NP_004334 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_004343 |
Tested Species Reactivity |
Human, Mouse |
Gene Symbol |
CALR |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
Application |
WB, IHC |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86% |
Image 1 | Human Brain
| WB Suggested Anti-CALR Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positive Control: Human brain |
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Image 2 | Mouse Small Intestine
| Host: Mouse Target Name: CALR Sample Tissue: Mouse Small Intestine Antibody Dilution: 1ug/ml |
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Image 3 | Thyroid
| Rabbit Anti-CALR antibody Catalog Number: ARP58880 Formalin Fixed Paraffin Embedded Tissue: Human Adult Thyroid Observed Staining: Cytoplasm in hepatocytes Primary Antibody Concentration: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 2.0 sec
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Image 4 | Human heart
| Rabbit Anti-CALR Antibody Catalog Number: ARP58880_P050 Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue Observed Staining: Cytoplasmic, plasma membrane in intercalated disc Primary Antibody Concentration: 1:100 Other Working Concentrations: 1:600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec |
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