PPP1CA Antibody - N-terminal region (ARP58652_P050)

• Name: PPP1CA Antibody - N-terminal region (ARP58652_P050)
• Protein Size (# AA): 341 amino acids
• Molecular Weight: 38kDa
• NCBI Gene Id: 5499
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Protein phosphatase 1, catalytic subunit, alpha isozyme
• Alias Symbols: PP1A, PP-1A, PPP1A, PP1alpha
• Peptide Sequence: Synthetic peptide located within the following region: MSDSEKLNLDSIIGRLLEGSRVLTPHCAPVQGSRPGKNVQLTENEIRGLC
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Lesage,B., (2007) Biochemistry 46 (31), 8909-8919
• Description of Target: PPP1CA is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory.The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Protein Interactions: HUWE1; PPP1R2P3; CSRNP2; CSRNP1; UBC; SUMO2; SUMO3; BRCA1; Kctd20; WWOX; PKM; SCPEP1; TMOD3; MAT2B; STK24; YWHAH; XPO1; TBCB; CAPNS1; ALDH7A1; ASNS; YAP1; LMTK2; RB1; POLR2A; GSK3B; MST1R; IKBKB; CHUK; CTDP1; TNFRSF1A; tat; PPP1R15A; PPP1R13B; PLCL2; CD2B
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-PPP1CA (ARP58652_P050) antibody is Catalog # AAP58652 (Previous Catalog # AAPP35789)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PPP1CA
• Uniprot ID: Q07161
• Protein Name: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
• Sample Type Confirmation:

  PPP1CA is supported by BioGPS gene expression data to be expressed in Jurkat

• Protein Accession #: NP_001008709
• Purification: Affinity Purified
• Nucleotide Accession #: NM_001008709
• Tested Species Reactivity: Human
• Gene Symbol: PPP1CA
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Image 1: HEK293, H1299
  
  WB Suggested Anti-PPP1CA Antibody
  Positive Control: Lane 1: 40ug HEK293 lysate Lane 2: 40ug H1299 lysate
  Primary Antibody Dilution : 1:1000
  Secondary Antibody : Goat anti-rabbit-HRP
  Secondry Antibody Dilution : 1:5000
  Submitted by: Jose Luis Rosa, Universitat de Barcelona
• Image 2: Human Jurkat
  
  WB Suggested Anti-PPP1CA Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:1562500
  Positive Control: Jurkat cell lysatePPP1CA is supported by BioGPS gene expression data to be expressed in Jurkat