| Product Number |
ARP58599_P050 |
| Product Page |
www.avivasysbio.com/bub3-antibody-n-terminal-region-arp58599-p050.html |
| Name |
BUB3 Antibody - N-terminal region (ARP58599_P050) |
| Protein Size (# AA) |
326 amino acids |
| Molecular Weight |
36kDa |
| NCBI Gene Id |
9184 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Budding uninhibited by benzimidazoles 3 homolog (yeast) |
| Alias Symbols |
BUB3L, hBUB3 |
| Peptide Sequence |
Synthetic peptide located within the following region: MTGSNEFKLNQPPEDGISSVKFSPNTSQFLLVSSWDTSVRLYDVPANSMR |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Vaclavicek,A., (2007) Breast Cancer Res. Treat. 106 (2), 205-213 |
| Description of Target |
BUB3 is required for kinetochore localization of BUB1.This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
| Protein Interactions |
BUB1B; BUB1; HUWE1; PSTPIP1; UBC; SUMO2; CDC20; NEDD8; SUMO1; RPA3; RPA2; RPA1; WBP4; CLIP1; EGFR; ZNF207; VCAM1; UBA1; THRA; RPL37A; ITGA4; FN1; NKAP; EXOC3; SUGT1; CPNE1; ANAPC4; SEPT2; TOP1; SMAD3; SMAD2; Mad2l1; COPS5; CUL3; SF3A2; ELAVL1; CDC27; HDGF |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-BUB3 (ARP58599_P050) antibody |
| Blocking Peptide |
For anti-BUB3 (ARP58599_P050) antibody is Catalog # AAP58599 (Previous Catalog # AAPP35736) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human BUB3 |
| Uniprot ID |
A6NJ42 |
| Protein Name |
Mitotic checkpoint protein BUB3 |
| Sample Type Confirmation |
BUB3 is strongly supported by BioGPS gene expression data to be expressed in HEK293T, PANC1 |
| Protein Accession # |
NP_001007794 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_001007793 |
| Tested Species Reactivity |
Human, Mouse |
| Gene Symbol |
BUB3 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Guinea Pig, Horse, Rabbit |
| Application |
WB, IF, IHC |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100% |
| Image 1 | Human PANC1
 | WB Suggested Anti-BUB3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: PANC1 cell lysate.BUB3 is strongly supported by BioGPS gene expression data to be expressed in PANC1 |
|
| Image 2 | Human HEK293T
 | Host: Rabbit
Target Name: BUB3
Sample Type: 293T
Antibody Dilution: 1.0ug/mlBUB3 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells |
|
| Image 3 | HCT116
 | Sample Type:
HCT116
Primary Antibody Dilution:
4 ug/ml
Secondary Antibody:
Anti-rabbit Alexa 546
Secondary Antibody Dilution:
2 ug/ml
Gene Name:
BUB3 |
|
| Image 4 | Mouse Liver
 | Host: Mouse
Target Name: BUB3
Sample Tissue: Mouse Liver
Antibody Dilution: 1ug/ml |
|
| Image 5 | Human heart
 | Rabbit Anti-BUB3 Antibody
Catalog Number: ARP58599_P050
Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
Observed Staining: Nucleus
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec |
|
