GAPDH Antibody - middle region (ARP58580_P050)

• Product Number: ARP58580_P050
• Name: GAPDH Antibody - middle region (ARP58580_P050)
• Protein Size (# AA): 335 amino acids
• Molecular Weight: 36kDa
• NCBI Gene Id: 2597
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Glyceraldehyde-3-phosphate dehydrogenase
• Alias Symbols: G3PD, GAPD, HEL-S-162eP
• Peptide Sequence: Synthetic peptide located within the following region: KAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNC
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Rinne,T., (2008) Hum. Mol. Genet. 17 (13), 1968-1977
• Description of Target: GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a
• Protein Interactions: FUS; HUWE1; UBC; SUMO2; SUMO3; STAU1; MDM2; GAPDH; ASB15; ASB16; RPA3; RPA2; RPA1; EED; SNCA; rev; HSPA9; HSPA1L; GAS7; TUBA1A; TUFM; RAP1GDS1; PPP5C; PPIB; YWHAG; TUBB; TUBA1C; PRPF40A; UBQLN1; DCPS; MAT2B; ACOT7; METAP2; FERMT2; GBP1; EEF1A1; TYMP; DFFA
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-GAPDH (ARP58580_P050) antibody is Catalog # AAP58580 (Previous Catalog # AAPP35526)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human GAPDH
• Uniprot ID: P04406
• Protein Name: Glyceraldehyde-3-phosphate dehydrogenase
• Sample Type Confirmation:

  There is BioGPS gene expression data showing that GAPDH is expressed in U87

• Protein Accession #: NP_002037
• Purification: Affinity Purified
• Nucleotide Accession #: NM_002046
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: GAPDH
• Predicted Species Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 90%; Rat: 100%
• Image 1: Human kidney
  
  WB Suggested Anti-GAPDH Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:1562500
  Positive Control: Human kidney
• Image 2: Mouse Kidney
  
  Host: Mouse
  Target Name: GAPDH
  Sample Tissue: Mouse Kidney
  Antibody Dilution: 1ug/ml
• Image 3: Human Raji
  
  WB Suggested Anti-GAPDH antibody Titration: 1 ug/mL
  Sample Type: Human Raji
• Image 4: Human U87
  
  Lanes:
  1) 10 ug
  2) 20 ug
  3) 40 ug U87 cell lysate
  Primary Antibody Dilution:
  1:1000
  Secondary Antibody:
  HRP-goat anti-rabbit IgG(H+L) antibody
  Secondary Antibody Dilution:
  1: 15000
  Gene Name:
  GAPDH
  Submitted by:
  Prof. Varda Shushan-Barmatz, Ben-Gurion University
  There is BioGPS gene expression data showing that GAPDH is expressed in U87