PSME1 Antibody - middle region (ARP58519_P050)

Data Sheet
 
Product Number ARP58519_P050
Product Page www.avivasysbio.com/psme1-antibody-middle-region-arp58519-p050.html
Name PSME1 Antibody - middle region (ARP58519_P050)
Protein Size (# AA) 250 amino acids
Molecular Weight 28kDa
Subunit 1
NCBI Gene Id 5720
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
Alias Symbols PA28A, IFI5111, REGalpha, PA28alpha, HEL-S-129m
Peptide Sequence Synthetic peptide located within the following region: KEKEERKKQQEKEDKDEKKKGEDEDKGPPCGPVNCNEKIVVLLQRLKPEI
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Lemaire,R., (2007) J. Proteome Res. 6 (11), 4127-4134
Description of Target The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Two transcripts encoding different isoforms have been identified.The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Two transcripts encoding different isoforms have been identified.
Protein Interactions HUWE1; PSME1; NAA10; PSMD14; SHFM1; UBC; PSME2; ADRM1; PSMA1; KIF5B; PARK2; BAG3; UBL7; PSRC1; PARVB; PHPT1; PRDX3; NUBP2; USP5; RBBP6; PSMD12; PSMD1; PSMC4; PSMC2; PSMB3; PSMB2; PSMB1; PSMA7; PSMA6; PSMA3; APP; OXCT1; CSE1L; GRB2; UBD; PSMD6; TUBG1; PSMB
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-PSME1 (ARP58519_P050) antibody
Blocking Peptide For anti-PSME1 (ARP58519_P050) antibody is Catalog # AAP58519 (Previous Catalog # AAPP34822)
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human PSME1
Uniprot ID Q06323
Protein Name Proteasome activator complex subunit 1
Protein Accession # NP_788955
Purification Affinity Purified
Nucleotide Accession # NM_176783
Tested Species Reactivity Human
Gene Symbol PSME1
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Pig, Rabbit, Yeast
Application WB
Predicted Homology Based on Immunogen Sequence Cow: 93%; Dog: 93%; Guinea Pig: 93%; Human: 100%; Mouse: 86%; Pig: 100%; Rabbit: 93%; Rat: 93%; Yeast: 83%
Image 1
Human Lung
WB Suggested Anti-PSME1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Human Lung
 

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