HNRPH1 Antibody - middle region (ARP58479_P050)

• Product Number: ARP58479_P050
• Name: HNRPH1 Antibody - middle region (ARP58479_P050)
• Protein Size (# AA): 449 amino acids
• Molecular Weight: 49 kDa
• NCBI Gene Id: 3187
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Heterogeneous nuclear ribonucleoprotein H1 (H)
• Description:
  View product citations for HNRPH1 Antibody - middle region (ARP58479_P050) on CiteAb
• Alias Symbols: HNRPH, HNRPH1, hnRNPH
• Peptide Sequence: Synthetic peptide located within the following region: FLNSTAGASGGAYEHRYVELFLNSTAGASGGAYGSQMMGGMGLSNQSSYG
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Olsen,J.V., (2006) Cell 127 (3), 635-648
• Description of Target: HNRPH1 belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. This protein has three repeats of quasi-RRM domains that bind to RNAs. It is very similar to the family member HNRPF. This gene is thought to be potentially involved in hereditary lymphedema type I phenotype.This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs. It is very similar to the family member HNRPF. This gene is thought to be potentially involved in hereditary lymphedema type I phenotype. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Protein Interactions: HUWE1; UBC; FUS; CEP76; CEP250; VCP; TP53; SUMO2; SUMO3; TUBGCP3; STAU1; MDM2; RPA3; RPA2; RPA1; ERG; ASB2; EED; rev; APBB1; AASDHPPT; IPO11; TNPO3; PEG10; PSMD9; PPP3CA; PPP2CA; PIN1; PARK2; PARP1; FBXO6; TARDBP; YWHAQ; ADRB2; IGSF8; ICAM1; CD81; PAN2; U
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-HNRNPH1 (ARP58479_P050) antibody is Catalog # AAP58479 (Previous Catalog # AAPP34947)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HNRPH1
• Uniprot ID: P31943
• Protein Name: Heterogeneous nuclear ribonucleoprotein H
• Sample Type Confirmation:

  HNRNPH1 is strongly supported by BioGPS gene expression data to be expressed in 721_B, Jurkat, MCF7

  HNRNPH1 is supported by BioGPS gene expression data to be expressed in HepG2

• Protein Accession #: NP_005511
• Purification: Affinity Purified
• Nucleotide Accession #: NM_005520
• Tested Species Reactivity: Human
• Gene Symbol: HNRNPH1
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Application: IF, IP, WB, IHC
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Image 1: Human Testis
  
  IHC Information: Paraffin embedded testis tissue, tested with an antibody Dilution of 5 ug/ml.
• Image 2: MCF-7
  
  Sample Type:
  MCF7 cells
  Primary Antibody Dilution:
  1:200
  Secondary Antibody:
  Anti-rabbit-FITC
  Secondary Antibody Dilution:
  1:500
  Color/Signal Descriptions:
  DAPI: Blue HNRNPH1: Green
  Gene Name:
  HNRPH1
  Submitted by:
  Anonymous.HNRNPH1 is strongly supported by BioGPS gene expression data to be expressed in MCF7
• Image 3: K562
  
  Amount and Sample Type:
  Lane 1: 5% InputLane 2: 5% SupLane 3: Normal IgGLane 4: hn-RNPH1 ppt. k562 sample
  IP Antibody:
  HNRPH1
  Amount of IP Antibody:
  
  Primary Antibody:
  HNRPH1
  Primary Antibody Dilution:
  1:4000
  Secondary Antibody:
  Anti-rabbit-HRP
  Secondary Antibody Dilution:
  1:5000
  Gene Name:
  HNRPH1
  Submitted by:
  Anonymous
• Image 4:
  
  25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.