DLD Antibody - middle region (ARP58455_P050)

• Product Number: ARP58455_P050
• Name: DLD Antibody - middle region (ARP58455_P050)
• Protein Size (# AA): 509 amino acids
• Molecular Weight: 54 kDa
• NCBI Gene Id: 1738
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Dihydrolipoamide dehydrogenase
• Alias Symbols: E3, LAD, DLDD, DLDH, GCSL, PHE3, OGDC-E3
• Peptide Sequence: Synthetic peptide located within the following region: AGEMVNEAALALEYGASCEDIARVCHAHPTLSEAFREANLAASFGKSINF
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Wang,Y.C., (2008) J. Biomed. Sci. 15 (1), 37-46
• Description of Target: DLD is the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Protein Interactions: HUWE1; FUS; UBC; SUMO1; NEDD8; MDM2; ASB15; PEPD; LDHA; CKB; ASS1; CNDP2; TNFAIP8; DPP3; RPL23; FN1; ATF2; MRPL11; UBAP2; MRPL37; MRPL13; MRPL42; GGH; UQCRC2; UQCRC1; MRPL23; NONO; NDUFB5; NDUFA9; ETFB; DLAT; DBT; AK2; SIRT7; SLC2A4; PSMD4; OGDH; DLD; PDH
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-DLD (ARP58455_P050) antibody is Catalog # AAP58455 (Previous Catalog # AAPP34562)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human DLD
• Uniprot ID: B2R5X0
• Protein Name: Dihydrolipoyl dehydrogenase, mitochondrial
• Sample Type Confirmation:

  DLD is supported by BioGPS gene expression data to be expressed in HeLa, Jurkat, MCF7

• Protein Accession #: NP_000099
• Purification: Affinity Purified
• Nucleotide Accession #: NM_000108
• Tested Species Reactivity: Human, Rat
• Gene Symbol: DLD
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 86%; Rabbit: 100%; Rat: 93%; Yeast: 86%; Zebrafish: 93%
• Image 1: Rat kidney
  
  DLD antibody - middle region (ARP58455_P050) validated by WB using Proximal kidney tubules purfied from cortex at 1:1000.
• Image 2: Human Fetal Kidney
  
  Host: Rabbit
  Target Name: DLD
  Sample Tissue: Human Fetal Kidney
  Antibody Dilution: 1.0ug/ml
• Image 3: Human Pineal Tissue
  
  Rabbit Anti-DLD Antibody
  Catalog Number: ARP58455_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue
  Observed Staining: Cytoplasmic in cell bodies and processes of pinealocytes
  Primary Antibody Concentration: 1:100
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 - 2.0 sec
  
• Image 4:
  
  25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 2 ug/mL of the antibody was used in this experiment.