| Product Number |
ARP58281_P050 |
| Product Page |
www.avivasysbio.com/h2afx-antibody-middle-region-arp58281-p050.html |
| Name |
H2AFX Antibody - middle region (ARP58281_P050) |
| Protein Size (# AA) |
143 amino acids |
| Molecular Weight |
16kDa |
| NCBI Gene Id |
3014 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
H2A histone family, member X |
| Alias Symbols |
H2A.X, H2A/X, H2AFX |
| Peptide Sequence |
Synthetic peptide located within the following region: ELNKLLGGVTIAQGGVLPNIQAVLLPKKTSATVGPKAPSGGKKATQASQE |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Description of Target |
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA |
| Protein Interactions |
UBC; HIST1H3A; USP17L2; UBD; RNF8; DDX21; XRCC6; NBN; NCL; HIST1H3B; ESR1; CENPA; TP53BP1; TOPORS; MDC1; BRCA1; ATR; ATM; PRKDC; CBX5; TRIM28; EGFR; BMI1; HIST2H3C; PML; XRCC5; UBB; TERF2; RNF2; SIRT7; CSNK2A1; ARRB2; ARRB1; PAXIP1; MRE11A; ATF2; MCPH1; C |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-H2AFX (ARP58281_P050) antibody |
| Blocking Peptide |
For anti-H2AFX (ARP58281_P050) antibody is Catalog # AAP58281 (Previous Catalog # AAPP32880) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human H2AFX |
| Uniprot ID |
P16104 |
| Protein Name |
Histone H2A.x |
| Sample Type Confirmation |
There is BioGPS gene expression data showing that H2AFX is expressed in NCIH226 |
| Protein Accession # |
NP_002096 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_002105 |
| Tested Species Reactivity |
Human |
| Gene Symbol |
H2AFX |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig |
| Application |
IF, IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 93%; Dog: 93%; Guinea Pig: 93%; Human: 100%; Mouse: 79%; Rat: 86% |
| Image 1 | MCF7
 | Sample Type:
MCF7
Primary Antibody Dilution:
4 ug/ml
Secondary Antibody:
Anti-rabbit Alexa 546
Secondary Antibody Dilution:
2 ug/ml
Gene Name:
H2AFX |
|
| Image 2 | Human NCIH226
 | WB Suggested Anti-H2AFX Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: NCI-H226 cell lysateThere is BioGPS gene expression data showing that H2AFX is expressed in NCIH226 |
|
| Image 3 | Human Tonsil
 | Immunohistochemistry with Tonsil tissue at an antibody concentration of 5ug/ml using anti-H2AFX antibody (ARP58281_P050) |
|
