H2AFX Antibody - N-terminal region (ARP58280_P050)

• Product Number: ARP58280_P050
• Name: H2AFX Antibody - N-terminal region (ARP58280_P050)
• Protein Size (# AA): 143 amino acids
• Molecular Weight: 16kDa
• NCBI Gene Id: 3014
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: H2A histone family, member X
• Alias Symbols: H2A.X, H2A/X, H2AFX
• Peptide Sequence: Synthetic peptide located within the following region: SGRGKTGGKARAKAKSRSSRAGLQFPVGRVHRLLRKGHYAERVGAGAPVY
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Scherthan,H., (2008) Biochem. Biophys. Res. Commun. 371 (4), 694-697
• Description of Target: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. H2AFX is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Protein Interactions: UBC; HIST1H3A; USP17L2; UBD; RNF8; DDX21; XRCC6; NBN; NCL; HIST1H3B; ESR1; CENPA; TP53BP1; TOPORS; MDC1; BRCA1; ATR; ATM; PRKDC; CBX5; TRIM28; EGFR; BMI1; HIST2H3C; PML; XRCC5; UBB; TERF2; RNF2; SIRT7; CSNK2A1; ARRB2; ARRB1; PAXIP1; MRE11A; ATF2; MCPH1; C
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-H2AFX (ARP58280_P050) antibody is Catalog # AAP58280 (Previous Catalog # AAPP32879)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human H2AFX
• Uniprot ID: P16104
• Protein Name: Histone H2A.x
• Publications:

  Wen, W. et al. MST1 promotes apoptosis through phosphorylation of histone H2AX. J. Biol. Chem. 285, 39108-16 (2010). 20921231

• Sample Type Confirmation:

  There is BioGPS gene expression data showing that H2AFX is expressed in HepG2

• Protein Accession #: NP_002096
• Purification: Affinity Purified
• Nucleotide Accession #: NM_002105
• Tested Species Reactivity: Human
• Gene Symbol: H2AFX
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 100%
• Image 1: Human HepG2
  
  WB Suggested Anti-H2AFX Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:312500
  Positive Control: HepG2 cell lysateThere is BioGPS gene expression data showing that H2AFX is expressed in HepG2