CENPH Antibody - middle region (ARP58266_P050)

• Product Number: ARP58266_P050
• Name: CENPH Antibody - middle region (ARP58266_P050)
• Protein Size (# AA): 247 amino acids
• Molecular Weight: 27kDa
• NCBI Gene Id: 64946
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Centromere protein H
• Alias Symbols: NNF1, PMF1
• Peptide Sequence: Synthetic peptide located within the following region: ESWDLEEKLLDIRKKRLQLKQASESKLLEIQTEKNKQKIDLDSMENSERI
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Liao,W.T., Clin. Cancer Res. 13 (2 PT 1), 508-514 (2007)
• Description of Target: Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. CENPH colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex.Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Protein Interactions: CENPH; CENPK; MTAP; NDC80; NUF2; SUGT1; UBC; VCP; CENPU; CENPN; CENPA; TRIM36; CENPP; CENPO; CENPI; ELAVL1; KIF2C; PMVK;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-CENPH (ARP58266_P050) antibody is Catalog # AAP58266 (Previous Catalog # AAPP32865)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CENPH
• Uniprot ID: Q9H3R5
• Protein Name: Centromere protein H
• Protein Accession #: NP_075060
• Purification: Affinity Purified
• Nucleotide Accession #: NM_022909
• Tested Species Reactivity: Human
• Gene Symbol: CENPH
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 79%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 93%; Pig: 100%; Rabbit: 86%; Rat: 93%
• Image 1: Human Liver
  
  WB Suggested Anti-CENPH Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:1562500
  Positive Control: Human Liver