HMGB2 Antibody - middle region (ARP58157_P050)

• Product Number: ARP58157_P050
• Name: HMGB2 Antibody - middle region (ARP58157_P050)
• Protein Size (# AA): 209 amino acids
• Molecular Weight: 24kDa
• NCBI Gene Id: 3148
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: High mobility group box 2
• Alias Symbols: HMG2
• Peptide Sequence: Synthetic peptide located within the following region: DREMKNYVPPKGDKKGKKKDPNAPKRPPSAFFLFCSEHRPKIKSEHPGLS
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: McCauley,M.J., (2007) J. Mol. Biol. 374 (4), 993-1004
• Description of Target: HMGB2 is a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. HMGB2 was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination.This gene encodes a member of the non-histone chromosomal high mobility group protein family. The proteins of this family are chromatin-associated and ubiquitously distributed in the nucleus of higher eukaryotic cells. In vitro studies have demonstrated that this protein is able to efficiently bend DNA and form DNA circles. These studies suggest a role in facilitating cooperative interactions between cis-acting proteins by promoting DNA flexibility. This protein was also reported to be involved in the final ligation step in DNA end-joining processes of DNA double-strand breaks repair and V(D)J recombination. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Protein Interactions: PKNOX1; UBC; EED; YWHAG; PGD; LDHB; CKB; HMGA1; GZMK; GZMA; CSNK1A1; FN1; SCAF8; APP; TFAP4; CDK2; BKRF1; Cebpb; ELAVL1; HMGB1; CHAF1A; CREBBP; SET; PGR; POU3F1; PRKDC; TP53; RAG1; POU5F1; POU2F2; POU2F1; APEX1; AR; NR3C1;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-HMGB2 (ARP58157_P050) antibody is Catalog # AAP58157 (Previous Catalog # AAPP32590)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human HMGB2
• Uniprot ID: P26583
• Protein Name: High mobility group protein B2
• Sample Type Confirmation:

  HMGB2 is supported by BioGPS gene expression data to be expressed in Jurkat

• Protein Accession #: NP_002120
• Purification: Affinity Purified
• Nucleotide Accession #: NM_002129
• Tested Species Reactivity: Human
• Gene Symbol: HMGB2
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit, Zebrafish
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 87%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 80%
• Image 1: Human NT-2
  
  Sample Type: 1. Human NT-2 cells (60ug)
  Primary Antibody Dilution: 2ug/ml
  Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience
  Secondary Antibody Dilution: 1: 20,000
  Image Submitted by: Yuzhi Chen
  University of Arkansas for Medical Science 
• Image 2: Human NT-2
  
  Sample Type: NT2 cells
  Red: Antibody
  Blue: DAPI
  Primary Dilution: 1ug/50ul antibody
  Secondary Antibody: Alexa goat anti-rabbit 594
  Image Submitted by: Yuzhi Chen, University of Arkansas for Medical Sciences  
• Image 3: Human brain
  
  Sample Type:
  Human brain stem cells
  Primary Antibody Dilution:
  1:500
  Secondary Antibody:
  Goat anti-rabbit Alexa-Fluor 594
  Secondary Antibody Dilution:
  1:1000
  Color/Signal Descriptions:
  HMGB2: Red DAPI:Blue
  Gene Name:
  HMGB2
  Submitted by:
  Dr. Yuzhi Chen, University of Arkansas for Medical Science
• Image 4: Human Jurkat
  
  WB Suggested Anti-HMGB2 Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:12500
  Positive Control: Jurkat cell lysateHMGB2 is supported by BioGPS gene expression data to be expressed in Jurkat