Product Number |
ARP58127_P050 |
Product Page |
www.avivasysbio.com/runx1t1-antibody-middle-region-arp58127-p050.html |
Name |
RUNX1T1 Antibody - middle region (ARP58127_P050) |
Protein Size (# AA) |
577 amino acids |
Molecular Weight |
64kDa |
NCBI Gene Id |
862 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Runt-related transcription factor 1; translocated to, 1 (cyclin D-related) |
Alias Symbols |
CDR, ETO, MTG8, AML1T1, ZMYND2, CBFA2T1, AML1-MTG8 |
Peptide Sequence |
Synthetic peptide located within the following region: CGSFCQHKDWEKHHHICGQTLQAQQQGDTPAVSSSVTPNSGAGSPMDTP |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Salat,D., (2008) Mol. Cell. Biol. 28 (10), 3502-3512 |
Description of Target |
RUNX1T1 is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Several transcript variants encoding multiple isoforms have been found for this gene. |
Protein Interactions |
EPS8; NECAB2; ABI3; GSE1; MID2; SPRY2; HOMER3; LPXN; ZMYM4; STX11; MEOX2; CCDC36; TRIM42; SPERT; CREB3L1; LZTS2; EFHC2; CPSF7; C19orf57; PRDM14; CBFA2T2; NCOR1; UBQLN4; ATN1; BCL6; ETS1; RUNX1; DNMT1; SPEN; RBPJ; UBE2E2; UBC; TRIM33; HDAC1; NCOR2; SIN3A; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-RUNX1T1 (ARP58127_P050) antibody |
Blocking Peptide |
For anti-RUNX1T1 (ARP58127_P050) antibody is Catalog # AAP58127 (Previous Catalog # AAPP32560) |
Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human RUNX1T1 |
Uniprot ID |
B2R6I9 |
Protein Name |
cDNA, FLJ92968, highly similar to Homo sapiens runt-related transcription factor 1; translocated to, 1 (cyclin D-related) (RUNX1T1), transcript variant 1, mRNA EMBL BAG35486.1 |
Protein Accession # |
NP_004340 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_004349 |
Tested Species Reactivity |
Human |
Gene Symbol |
RUNX1T1 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit, Zebrafish |
Application |
WB, IHC |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 93%; Pig: 100%; Rabbit: 100%; Rat: 86%; Zebrafish: 93% |
Image 1 | Human 293T
| WB Suggested Anti-RUNX1T1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positive Control: 293T cell lysate |
|
Image 2 | Human Liver Tissue
| RUNX1T1 antibody - middle region (ARP58127_P050)
Catalog Number: ARP58127_P050
Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue
Observed Staining: Nucleus in hepatocytes
Primary Antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec |
|