RUNX1T1 Antibody - middle region (ARP58127_P050)

Data Sheet
 
Product Number ARP58127_P050
Product Page www.avivasysbio.com/runx1t1-antibody-middle-region-arp58127-p050.html
Name RUNX1T1 Antibody - middle region (ARP58127_P050)
Protein Size (# AA) 577 amino acids
Molecular Weight 64kDa
NCBI Gene Id 862
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Runt-related transcription factor 1; translocated to, 1 (cyclin D-related)
Alias Symbols CDR, ETO, MTG8, AML1T1, ZMYND2, CBFA2T1, AML1-MTG8
Peptide Sequence Synthetic peptide located within the following region: CGSFCQHKDWEKHHHICGQTLQAQQQGDTPAVSSSVTPNSGAGSPMDTP
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Salat,D., (2008) Mol. Cell. Biol. 28 (10), 3502-3512
Description of Target RUNX1T1 is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene is a putative zinc finger transcription factor and oncoprotein. In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Several transcript variants encoding multiple isoforms have been found for this gene.
Protein Interactions EPS8; NECAB2; ABI3; GSE1; MID2; SPRY2; HOMER3; LPXN; ZMYM4; STX11; MEOX2; CCDC36; TRIM42; SPERT; CREB3L1; LZTS2; EFHC2; CPSF7; C19orf57; PRDM14; CBFA2T2; NCOR1; UBQLN4; ATN1; BCL6; ETS1; RUNX1; DNMT1; SPEN; RBPJ; UBE2E2; UBC; TRIM33; HDAC1; NCOR2; SIN3A;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-RUNX1T1 (ARP58127_P050) antibody
Blocking Peptide For anti-RUNX1T1 (ARP58127_P050) antibody is Catalog # AAP58127 (Previous Catalog # AAPP32560)
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human RUNX1T1
Uniprot ID B2R6I9
Protein Name cDNA, FLJ92968, highly similar to Homo sapiens runt-related transcription factor 1; translocated to, 1 (cyclin D-related) (RUNX1T1), transcript variant 1, mRNA EMBL BAG35486.1
Protein Accession # NP_004340
Purification Affinity Purified
Nucleotide Accession # NM_004349
Tested Species Reactivity Human
Gene Symbol RUNX1T1
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit, Zebrafish
Application WB, IHC
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 93%; Pig: 100%; Rabbit: 100%; Rat: 86%; Zebrafish: 93%
Image 1
Human 293T
WB Suggested Anti-RUNX1T1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: 293T cell lysate
Image 2
Human Liver Tissue
RUNX1T1 antibody - middle region (ARP58127_P050)
Catalog Number: ARP58127_P050
Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue
Observed Staining: Nucleus in hepatocytes
Primary Antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
 

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