SIAH2 antibody - N-terminal region (ARP58071_P050)

• Name: SIAH2 antibody - N-terminal region (ARP58071_P050)
• Protein Size (# AA): 324 amino acids
• Molecular Weight: 35 kDa
• NCBI Gene Id: 6478
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Siah E3 ubiquitin protein ligase 2
• Alias Symbols: hSiah2
• Peptide Sequence: Synthetic peptide located within the following region: SRPSSTGPSANKPCSKQPPPQPQHTPSPAAPPAAATISAAGPGSSAVPAA
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Rott,R., (2008) J. Biol. Chem. 283 (6), 3316-3328
• Description of Target: SIAH2 is a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in regulating cellular response to hypoxia.This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in regulating cellular response to hypoxia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Protein Interactions: EGLN3; XAF1; HIPK2; UBE2D1; UBE2L6; SIAH2; UBE2D3; UBC; PPP1R13B; TP53BP2; NFE2L2; USP19; AR; UBE2I; STAT3; MYD88; EEF1D; CCBE1; SETD2; HIPK3; TNK2; KAT5; KAT2B; UBE2D2; TPX2; PML; ATN1; PSMD4; PPP1R12A; EGLN2; SH3RF1; EGLN1; DYRK2; PPP1CC; AFF1; DCC; CEB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation:

  WB	Y
  SPR	Y
  YCHAROS

• Blocking Peptide: For anti-SIAH2 (ARP58071_P050) antibody is Catalog # AAP58071 (Previous Catalog # AAPP32494)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SIAH2
• Uniprot ID: O43255
• Protein Name: E3 ubiquitin-protein ligase SIAH2
• Sample Type Confirmation:

  SIAH2 is supported by BioGPS gene expression data to be expressed in HepG2, MCF7

• Protein Accession #: NP_005058
• Purification: Affinity Purified
• Nucleotide Accession #: NM_005067
• Tested Species Reactivity: Human
• Gene Symbol: SIAH2
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Pig
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 93%; Dog: 92%; Human: 100%; Mouse: 80%; Pig: 93%; Rat: 80%
• Image 1: Human HeLa
  
  WB Suggested Anti-SIAH2 Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:1562500
  Positive Control: Hela cell lysate
• Image 2: Human
  
  Lanes:
  1: 50ug HEK293T lysate, 2: 50ug A549 lysate
  Primary Antibody Dilution:
  1:1000
  Secondary Antibody:
  Anti-rabbit-HRP
  Secondary Antibody Dilution:
  1:5000
  Gene Name:
  SIAH2
  Submitted by:
  Peter Brand & Dr. Oliver Krammer, Institute for Biotechnology and Biophysics, Center for Molecular Biomedicine, Friedrich-Schiller University, Jena
  
• Image 3: Hela
  
  Host: Rabbit
  Target Name: SIAH2
  Sample Type: Hela
  Lane A: Primary Antibody
  Lane B: Primary Antibody + Blocking Peptide
  Primary Antibody Concentration: 1ug/ml
  Peptide Concentration: 5ug/ml
  Lysate Quantity: 25ug/lane/lane
  Gel Concentration: 0.12
• Image 4:
  
  Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 0.5, 5, and 50 ug/mL. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for either two or three concentrations were averaged and results and standard deviation are shown.