| Product Number |
ARP56652_P050 |
| Product Page |
www.avivasysbio.com/sgk1-antibody-n-terminal-region-arp56652-p050.html |
| Name |
SGK1 Antibody - N-terminal region (ARP56652_P050) |
| Protein Size (# AA) |
431 amino acids |
| Molecular Weight |
49kDa |
| NCBI Gene Id |
6446 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Serum/glucocorticoid regulated kinase 1 |
| Alias Symbols |
SGK |
| Peptide Sequence |
Synthetic peptide located within the following region: ANPSPPPSPSQQINLGPSSNPHAKPSDFHFLKVIGKGSFGKVLLARHKAE |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Pew,T., (2008) Endocrinology 149 (5), 2637-2645 |
| Description of Target |
This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell sur |
| Protein Interactions |
AKT1S1; YWHAQ; YWHAZ; MAPT; MTOR; CDKN1B; RPTOR; NEDD4L; Tmem109; Rabac1; GADD45G; UBC; MAGEA1; HSP90AA1; TMEM184C; WNK1; WNK2; WNK4; RAF1; PDPK1; NEDD4; TSC22D3; STUB1; HSPA4; ELAVL1; COMMD1; SYVN1; SCNN1B; RICTOR; PDK1; YPK1; IKBKG; CAMKK1; KCNJ1; GSK3A |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-SGK1 (ARP56652_P050) antibody |
| Blocking Peptide |
For anti-SGK1 (ARP56652_P050) antibody is Catalog # AAP56652 (Previous Catalog # AAPP39409) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human SGK1 |
| Uniprot ID |
O00141 |
| Protein Name |
Serine/threonine-protein kinase Sgk1 |
| Sample Type Confirmation |
SGK1 is supported by BioGPS gene expression data to be expressed in HepG2 |
| Protein Accession # |
NP_005618 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_005627 |
| Tested Species Reactivity |
Human, Mouse |
| Gene Symbol |
SGK1 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
| Application |
WB, IHC |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 92% |
| Image 1 | Mouse renal epithelial
 | Lanes:
Lane 1: 30ug mouse renal epithelial lysate
Lane 2: 30ug mouse renal epithelial lysate
Lane 3: 30ug mouse renal epithelial lysate
Primary Antibody Dilution:
1:500
Secondary Antibody:
Anti-rabbit-HRP
Secondary Antibody Dilution:
1:2500
Gene Name:
SGK1
Submitted by:
Dr. JUNMING FAN,ECU
|
|
| Image 2 | Human liver tissue
 | Rabbit Anti-SGK1 Antibody
Catalog Number: ARP56652_P050
Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue
Observed Staining: Cytoplasm and plasma membrane in sinusoids
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec |
|
| Image 3 | Human Pineal Tissue
 | Rabbit Anti-SGK1 Antibody
Catalog Number: ARP56652_P050
Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue
Observed Staining: Cytoplasmic in cell bodies and processes of pinealocytes
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1/600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
|
|
| Image 4 | Human HepG2
 | WB Suggested Anti-SGK1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: HepG2 cell lysateSGK1 is supported by BioGPS gene expression data to be expressed in HepG2 |
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