PKP2 Antibody - N-terminal region (ARP56587_P050)

• Product Number: ARP56587_P050
• Name: PKP2 Antibody - N-terminal region (ARP56587_P050)
• Protein Size (# AA): 837 amino acids
• Molecular Weight: 93kDa
• NCBI Gene Id: 5318
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Plakophilin 2
• Alias Symbols: ARVD9
• Peptide Sequence: Synthetic peptide located within the following region: HPLRRLEISPDSSPERAHYTHSDYQYSQRSQAGHTLHHQESRRAALLVPP
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Krangel,M.S., (2008) J. Cell Biol. 181 (4), 605-613
• Description of Target: This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the
• Protein Interactions: NDEL1; MTUS2; CEP250; DROSHA; BMI1; EED; RNF2; YWHAB; CLK2; YWHAE; WHSC1; UBC; SMAD9; YWHAQ; CUL3; MARK3; POLR3A; YWHAG; PKP2; JUP; KRT18; KRT5; DSG2; DSG1; DSP; DSC2; DSC1; CTNNB1; GTF2B; SFN;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-PKP2 (ARP56587_P050) antibody is Catalog # AAP56587 (Previous Catalog # AAPP39244)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PKP2
• Uniprot ID: A0AV37
• Protein Name: Plakophilin-2
• Sample Type Confirmation:

  PKP2 is supported by BioGPS gene expression data to be expressed in COLO205

• Protein Accession #: NP_001005242
• Purification: Affinity Purified
• Nucleotide Accession #: NM_001005242
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: PKP2
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Horse
• Application: WB, IHC
• Predicted Homology Based on Immunogen Sequence: Cow: 77%; Dog: 79%; Horse: 86%; Human: 100%; Mouse: 86%; Rat: 77%
• Image 1: Human COLO205
  
  WB Suggested Anti-PKP2 Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:312500
  Positive Control: COLO205 cell lysatePKP2 is supported by BioGPS gene expression data to be expressed in COLO205
• Image 2: mouse keratinocyte
  
  WB Suggested Anti-PKP2 Antibody
  Positive Control: Lane1-4: 20ug mouse keratinocyte lysate
  Primary Antibody Dilution : 1:1000
  Secondary Antibody : Anti-rabbit-HRP
  Secondry Antibody Dilution : 1:6000
  Submitted by: Jiangli Chen and Peter Koch, University of Colorado Denver
• Image 3: Human heart
  
  Rabbit Anti-PKP2 Antibody
  Catalog Number: ARP56587_P050
  Formalin Fixed Paraffin Embedded Tissue: Human heart Tissue
  Observed Staining: Cytoplasmic
  Primary Antibody Concentration: N/A
  Other Working Concentrations: 1:600
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 - 2.0 sec
• Image 4: A549 Cell Lysate, U937 Cell Lysate
  
  Host: Rabbit
  Target: PKP2
  Positive control (+): A549 Cell Lysate (A549)
  Negative control (-): U937 Cell Lysate (N31)
  Antibody concentration: 1ug/ml