FANCL Antibody - middle region (ARP56321_P050)

• Product Number: ARP56321_P050
• Name: FANCL Antibody - middle region (ARP56321_P050)
• Protein Size (# AA): 375 amino acids
• Molecular Weight: 43 kDa
• NCBI Gene Id: 55120
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Fanconi anemia, complementation group L
• Alias Symbols: POG, PHF9, FAAP43
• Peptide Sequence: Synthetic peptide located within the following region: ASGREHLITLKLKAKYPAESPDYFVDFPVPFCASWTPQVNSPQSSLISIY
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Bethke,L., (2008) J. Natl. Cancer Inst. 100 (4), 270-276
• Description of Target: The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FAN
• Protein Interactions: CCDC36; STRA13; RBM45; SSX2IP; RIMBP3; LZTS2; DOCK8; EIF4ENIF1; CHCHD3; IKZF3; TFCP2; KIFC3; GRN; C1orf86; C17orf70; FANCM; UBC; FANCG; FANCF; UBE2W; UBE2T; FANCI; FANCD2; UBD; FANCA; CTNNB1; FANCB; FANCL; C19orf40; FANCC; HES1; APITD1; PCNA; GGN; BMPR1B;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation:

  WB	Y
  SPR
  YCHAROS

• Blocking Peptide: For anti-FANCL (ARP56321_P050) antibody is Catalog # AAP56321 (Previous Catalog # AAPP38333)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human FANCL
• Uniprot ID: Q9NW38
• Protein Name: E3 ubiquitin-protein ligase FANCL
• Sample Type Confirmation:

  FANCL is supported by BioGPS gene expression data to be expressed in HEK293

• Protein Accession #: NP_001108108
• Purification: Affinity Purified
• Nucleotide Accession #: NM_001114636
• Tested Species Reactivity: Human
• Gene Symbol: FANCL
• Predicted Species Reactivity: Human, Dog, Rabbit
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Dog: 93%; Human: 100%; Rabbit: 86%
• Image 1: Human Hela Whole Cell
  
  Host: Rabbit
  Target Name: FANCL
  Sample Tissue: Human Hela Whole Cell
  Antibody Dilution: 1ug/ml
• Image 2: Human Hela Whole Cell
  
  Host: Rabbit
  Target Name: FANCL
  Sample Tissue: Human Hela Whole Cell
  Antibody Dilution: 2ug/ml
• Image 3: Human HEK-293
  
  Lanes:
  Lane 1: 7ug HEK293 cytoplasmic lysate
  2: 7ug HEK293 nuclei lysate
  Primary Antibody Dilution:
  1:1000
  Secondary Antibody:
  Anti-rabbit-HRP
  Secondary Antibody Dilution:
  1:3000
  Gene Name:
  FANCL
  Submitted by:
  Seiji Masuda, Kitashirakawa Oiwakecho, Kyoto University
  FANCL is supported by BioGPS gene expression data to be expressed in HEK293
• Image 4: Human Pineal Tissue
  
  Rabbit Anti-FANCL Antibody
  Catalog Number: ARP56321_P050
  Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue
  Observed Staining: Cytoplasmic and nuclear in in pinealocytes
  Primary Antibody Concentration: 1:100
  Secondary Antibody: Donkey anti-Rabbit-Cy3
  Secondary Antibody Concentration: 1:200
  Magnification: 20X
  Exposure Time: 0.5 - 2.0 sec