Product Number |
ARP55596_P050 |
Product Page |
www.avivasysbio.com/med19-antibody-middle-region-arp55596-p050.html |
Name |
MED19 Antibody - middle region (ARP55596_P050) |
Protein Size (# AA) |
244 amino acids |
Molecular Weight |
26kDa |
NCBI Gene Id |
219541 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
mediator complex subunit 19 |
Alias Symbols |
LCMR1, DT2P1G7, MED19AS |
Peptide Sequence |
Synthetic peptide located within the following region: LPGSHDNSSLRSLIEKPPILSSSFNPITGTMLAGFRLHTGPLPEQCRLMH |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Description of Target |
MED19 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II . |
Protein Interactions |
MED25; CDK19; MED6; MED13; MED12; MED26; CDK8; CCNC; MED20; MED7; MED27; MED17; MED23; MED21; MED14; MED1; POLR2L; POLR2K; POLR2J; POLR2I; POLR2H; POLR2G; POLR2F; POLR2E; POLR2D; POLR2C; POLR2B; POLR2A; MSX1; MED29; MED9; MED18; MED15; MED31; MED4; AFF4; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-MED19 (ARP55596_P050) antibody |
Blocking Peptide |
For anti-MED19 (ARP55596_P050) antibody is Catalog # AAP55596 |
Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of Human MED19 |
Uniprot ID |
A0JLT2 |
Protein Name |
Mediator of RNA polymerase II transcription subunit 19 |
Protein Accession # |
NP_703151 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_153450 |
Tested Species Reactivity |
Human |
Gene Symbol |
MED19 |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
Application |
WB, CHIP |
Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 92% |
Image 1 | Human Jurkat
| Host: Rabbit Target Name: MED19 Sample Type: Jurkat Whole cell lysates Antibody Dilution: 1.0ug/ml |
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Image 2 | HCT116
| Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.
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Image 3 | Rat testis, Rat kidney
| Host: Rabbit Target: MED19 Positive control (+): Rat testis (R-TE) Negative control (-): Rat kidney (R-KI) Antibody concentration: 1ug/ml |
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Image 4 |
| Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown. |
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