FBXW8 Antibody - middle region (ARP55585_P050)

• Product Number: ARP55585_P050
• Name: FBXW8 Antibody - middle region (ARP55585_P050)
• Protein Size (# AA): 598 amino acids
• Molecular Weight: 67kDa
• NCBI Gene Id: 26259
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: F-box and WD repeat domain containing 8
• Alias Symbols: FBW6, FBW8, FBX29, FBXW6, FBXO29
• Peptide Sequence: Synthetic peptide located within the following region: MNQKLWEVYSGHPVQHISFSSHSLITANVPYQTVMRNADLDSFTTHRRHR
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Tsutsumi,T., (2008) Mol. Cell. Biol. 28 (2), 743-751
• Description of Target: FBXW8 is a member of the F-box protein family, members of which are characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. FBXW8 contains a WD-40 domain, in addition to an F-box motif, so it belongs to the Fbw class.This gene encodes a member of the F-box protein family, members of which are characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene contains a WD-40 domain, in addition to an F-box motif, so it belongs to the Fbw class. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene.
• Protein Interactions: MAP4K1; CUL1; TBC1D3; CUL7; UBE2D3; SKP1; IRS1; REST; CCND1; CUL4A; DDB1; UBC; CCDC8; GORASP1; OBSL1; CCT6B; CCT2; CCT7; TRIP13; PDCD5; CUL2; VBP1; CCT3; PFDN5; NFKBIA; CCT6A; BCL3; BTRC; SKP2; HSP90AA1; HSPA4; FBXO4; FBXW8; FBXW2; MYC; RBX1; UBI4; EP300;
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Blocking Peptide: For anti-FBXW8 (ARP55585_P050) antibody is Catalog # AAP55585 (Previous Catalog # AAPP34146)
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human FBXW8
• Uniprot ID: Q8N3Y1
• Protein Name: F-box/WD repeat-containing protein 8
• Publications:

  Lin, P. et al. Fbxw8 is involved in the proliferation of human choriocarcinoma JEG-3 cells. Mol. Biol. Rep. 38, 1741-7 (2011). 20878477

• Protein Accession #: NP_699179
• Purification: Affinity Purified
• Nucleotide Accession #: NM_153348
• Tested Species Reactivity: Human
• Gene Symbol: FBXW8
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Application: WB
• Predicted Homology Based on Immunogen Sequence: Cow: 83%; Dog: 92%; Guinea Pig: 83%; Horse: 92%; Human: 100%; Mouse: 92%; Rabbit: 100%; Rat: 92%
• Image 1: Human HepG2
  
  WB Suggested Anti-FBXW8 Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:1562500
  Positive Control: HepG2 cell lysate