SENP3 Antibody - N-terminal region (ARP55322_P050)

• Product Number: ARP55322_P050
• Name: SENP3 Antibody - N-terminal region (ARP55322_P050)
• Protein Size (# AA): 574 amino acids
• Molecular Weight: 65kDa
• NCBI Gene Id: 26168
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: SUMO1/sentrin/SMT3 specific peptidase 3
• Alias Symbols: SSP3, Ulp1, SMT3IP1
• Peptide Sequence: Synthetic peptide located within the following region: PPPKPRLKSGGGFGPDPGSGTTVPARRLPVPRPSFDASASEEEEEEEEEE
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Haindl,M., (2008) EMBO Rep. 9 (3), 273-279
• Description of Target: SENP3 is a protease that releases SUMO2 and SUMO3 monomers from sumoylated substrates, but has only weak activity against SUMO1 conjugates. SENP3 deconjugates SUMO2 from MEF2D, which increases its transcriptional activation capability.
• Protein Interactions: HIF1A; EP300; UBC; SUMO2; WDR5; ASH2L; KMT2D; RBBP5; KMT2A; MEN1; RNF2; HECW2; SOX2; LAS1L; PELP1; CDK6; CDK4; MOV10; CHCHD4; DHX15; ILF3; TRIM63; TRIM54; USP21; USP15; CDKN2A; RPS18; RPS11; RPS8; RPS4X; RPLP0; NPM1; PIDD1; NR1H2; TP53; MDM2; STUB1; HSP90
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Other Applications Image 1 Data:

  Sample Type: 1. HeLa cell extract (200ug)
  2. HeLa cell extract (200ug) incubated with HA-SUMO2-VME
  *HA-S2-VME-SENP3
  Primary Dilution: 1:1000
  Secondary Antibody: alkaline phosphatase-conjugated anti-rabbit
  Secondary Dilution: 1:1000
  Image Submitted by: Andreia Carvalho
  IBMC-OBF, Portugal 

  
  

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• Blocking Peptide: For anti-SENP3 (ARP55322_P050) antibody is Catalog # AAP55322 (Previous Catalog # AAPP33153)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SENP3
• Uniprot ID: Q9H4L4
• Protein Name: Sentrin-specific protease 3
• Sample Type Confirmation:

  SENP3 is strongly supported by BioGPS gene expression data to be expressed in 721_B. HeLa

• Protein Accession #: NP_056485
• Purification: Affinity Purified
• Nucleotide Accession #: NM_015670
• Tested Species Reactivity: Human, Rat
• Gene Symbol: SENP3
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
• Application: IF, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
• Image 1: Human HeLa
  
  Sample Type: 1. HeLa cell extract (200ug)Primary Dilution: 1:1000Secondary Antibody: alkaline phosphatase-conjugated anti-rabbitSecondary Dilution: 1:1000Image Submitted by: Andreia CarvalhoIBMC-OBF, Portugal.SENP3 is strongly supported by BioGPS gene expression data to be expressed in HeLa
• Image 2: Human HeLa
  
  Sample Type: 1. HeLa cell extract (200ug)2. HeLa cell extract (200ug) incubated with HA-SUMO2-VME*HA-S2-VME-SENP3Primary Dilution: 1:1000Secondary Antibody: alkaline phosphatase-conjugated anti-rabbitSecondary Dilution: 1:1000Image Submitted by: Andreia CarvalhoIBMC-OBF, Portugal.SENP3 is strongly supported by BioGPS gene expression data to be expressed in HeLa
• Image 3: Rat
  
  Sample Type: 1. Rat Liver (200ug)
  2. Rat Brain (200ug)
  3. Rat Testis (200ug)
  4. Rat Kidney (200ug)
  5.Rat Liver nuclei (-) no HA-SUMO2-WME
  6.Rat Liver nuclei (+) incubated with 100 ng HA-SUMO2-WME
  Primary Dilution: 1:1000
  Secondary Antibody: alkaline phosphatase-conjugated anti-rabbit
  Secondary Dilution: 1:1000
  Image Submitted by: Andreia Carvalho
  IBMC-OBF, PortugalSee Customer Feedback tab for detailed information.
  
  
• Image 4: Human HeLa
  
  SENP3 antibody - N-terminal region (ARP55322_P050) in HeLa cells using Immunofluorescence.
  Antibody concentration: 2 ug/ml
  Data submitted by: Michael Matunis, Johns Hopkins University
  SENP3 is strongly supported by BioGPS gene expression data to be expressed in HeLa
• Image 5: Human 721_B
  
  WB Suggested Anti-SENP3 Antibody Titration: 0.2-1 ug/ml
  ELISA Titer: 1:1562500
  Positive Control: 721_B cell lysateSENP3 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells