Product Number |
ARP54777_P050 |
Product Page |
www.avivasysbio.com/ldha-antibody-middle-region-arp54777-p050.html |
Name |
LDHA Antibody - middle region (ARP54777_P050) |
Protein Size (# AA) |
332 amino acids |
Molecular Weight |
37kDa |
NCBI Gene Id |
3939 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
lactate dehydrogenase A |
Alias Symbols |
LDHM, GSD11, PIG19, HEL-S-133P |
Peptide Sequence |
Synthetic peptide located within the following region: PLSCHGWVLGEHGDSSVPVWSGMNVAGVSLKTLHPDLGTDKDKEQWKEVH |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Listerman,I., PLoS Genet. 3 (11), E212 (2007) |
Description of Target |
The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. |
Protein Interactions |
MAPK10; LDHB; FUS; HUWE1; ISG15; UBC; SUMO2; SUMO3; STAU1; RPA3; RPA2; RPA1; ASB9; RNF2; CNDP2; NAGK; C11orf58; NDRG1; BZW1; KYNU; TCEA1; SORD; SMS; RPS6KA1; PGD; PAFAH1B3; GSR; PDIA3; GNAI3; DLD; CSE1L; LDHAL6B; FTO; BCCIP; XRN1; FBXO6; UPF2; YWHAQ; METT |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-LDHA (ARP54777_P050) antibody |
Blocking Peptide |
For anti-LDHA (ARP54777_P050) antibody is Catalog # AAP54777 (Previous Catalog # AAPP31572) |
Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human LDHA |
Uniprot ID |
P00338 |
Protein Name |
L-lactate dehydrogenase A chain |
Sample Type Confirmation |
LDHA is supported by BioGPS gene expression data to be expressed in HeLa |
Protein Accession # |
NP_005557 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_005566 |
Tested Species Reactivity |
Human |
Gene Symbol |
LDHA |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Zebrafish |
Application |
IHC, WB, IP |
Predicted Homology Based on Immunogen Sequence |
Cow: 79%; Dog: 86%; Goat: 79%; Guinea Pig: 79%; Horse: 79%; Human: 100%; Mouse: 86%; Rabbit: 86%; Rat: 79%; Zebrafish: 92% |
Image 1 | HEK293 Whole Cell Lysate
| LDHA was immunoprecipitated from 2 mg HEK293 Whole Cell Lysate with ARP54777_P050 with 1:200 dilution. Western blot was performed using ARP54777_P050 at 1/1000 dilution. Lane 1: Control IP in HEK293 Whole Cell Lysate. Lane 2: LDHA IP with ARP54777_P050 in HEK293 Whole Cell Lysate. Lane 3: Input of HEK293 Whole Cell Lysate. |
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Image 2 | Human Bronchial Epithelial Tissue
| Rabbit Anti-LDHA Antibody Catalog Number: ARP54777_P050 Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue Observed Staining: Cytoplasmic Primary Antibody Concentration: 1:100 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec
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Image 3 | Human Jurkat, MCF7, 721_B
| Host: Rabbit Target Name: LDHA Sample Tissue: Human Jurkat, MCF7, 721_B Antibody Dilution: 1.0ug/ml |
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