LDHA Antibody - middle region (ARP54777_P050)

Data Sheet
 
Product Number ARP54777_P050
Product Page www.avivasysbio.com/ldha-antibody-middle-region-arp54777-p050.html
Name LDHA Antibody - middle region (ARP54777_P050)
Protein Size (# AA) 332 amino acids
Molecular Weight 37kDa
NCBI Gene Id 3939
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name lactate dehydrogenase A
Alias Symbols LDHM, GSD11, PIG19, HEL-S-133P
Peptide Sequence Synthetic peptide located within the following region: PLSCHGWVLGEHGDSSVPVWSGMNVAGVSLKTLHPDLGTDKDKEQWKEVH
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Listerman,I., PLoS Genet. 3 (11), E212 (2007)
Description of Target The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.
Protein Interactions MAPK10; LDHB; FUS; HUWE1; ISG15; UBC; SUMO2; SUMO3; STAU1; RPA3; RPA2; RPA1; ASB9; RNF2; CNDP2; NAGK; C11orf58; NDRG1; BZW1; KYNU; TCEA1; SORD; SMS; RPS6KA1; PGD; PAFAH1B3; GSR; PDIA3; GNAI3; DLD; CSE1L; LDHAL6B; FTO; BCCIP; XRN1; FBXO6; UPF2; YWHAQ; METT
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-LDHA (ARP54777_P050) antibody
Blocking Peptide For anti-LDHA (ARP54777_P050) antibody is Catalog # AAP54777 (Previous Catalog # AAPP31572)
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human LDHA
Uniprot ID P00338
Protein Name L-lactate dehydrogenase A chain
Sample Type Confirmation

LDHA is supported by BioGPS gene expression data to be expressed in HeLa

Protein Accession # NP_005557
Purification Affinity Purified
Nucleotide Accession # NM_005566
Tested Species Reactivity Human
Gene Symbol LDHA
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Zebrafish
Application IHC, WB, IP
Predicted Homology Based on Immunogen Sequence Cow: 79%; Dog: 86%; Goat: 79%; Guinea Pig: 79%; Horse: 79%; Human: 100%; Mouse: 86%; Rabbit: 86%; Rat: 79%; Zebrafish: 92%
Image 1
HEK293 Whole Cell Lysate
LDHA was immunoprecipitated from 2 mg HEK293 Whole Cell Lysate with ARP54777_P050 with 1:200 dilution. Western blot was performed using ARP54777_P050 at 1/1000 dilution.
Lane 1: Control IP in HEK293 Whole Cell Lysate.
Lane 2: LDHA IP with ARP54777_P050 in HEK293 Whole Cell Lysate.
Lane 3: Input of HEK293 Whole Cell Lysate.
Image 2
Human Bronchial Epithelial Tissue
Rabbit Anti-LDHA Antibody
Catalog Number: ARP54777_P050
Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue
Observed Staining: Cytoplasmic
Primary Antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
Image 3
Human Jurkat, MCF7, 721_B
Host: Rabbit
Target Name: LDHA
Sample Tissue: Human Jurkat, MCF7, 721_B
Antibody Dilution: 1.0ug/ml
 

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