LDHA Antibody - N-terminal region (ARP54776_P050)

• Product Number: ARP54776_P050
• Name: LDHA Antibody - N-terminal region (ARP54776_P050)
• Protein Size (# AA): 332 amino acids
• Molecular Weight: 37 kDa
• NCBI Gene Id: 3939
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: lactate dehydrogenase A
• Alias Symbols: LDHM, GSD11, PIG19, HEL-S-133P
• Peptide Sequence: Synthetic peptide located within the following region: ATLKDQLIYNLLKEEQTPQNKITVVGVGAVGMACAISILMKDLADELALV
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reference: Listerman,I., PLoS Genet. 3 (11), E212 (2007)
• Description of Target: The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.
• Protein Interactions: MAPK10; LDHB; FUS; HUWE1; ISG15; UBC; SUMO2; SUMO3; STAU1; RPA3; RPA2; RPA1; ASB9; RNF2; CNDP2; NAGK; C11orf58; NDRG1; BZW1; KYNU; TCEA1; SORD; SMS; RPS6KA1; PGD; PAFAH1B3; GSR; PDIA3; GNAI3; DLD; CSE1L; LDHAL6B; FTO; BCCIP; XRN1; FBXO6; UPF2; YWHAQ; METT
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation:

  WB	Y
  SPR	Y
  YCHAROS

• Additional Information: IHC Information: Paraffin embedded liver tissue, tested with an antibody dilution of 2.5 ug/ml.
• Blocking Peptide: For anti-LDHA (ARP54776_P050) antibody is Catalog # AAP54776 (Previous Catalog # AAPP31571)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human LDHA
• Uniprot ID: P00338
• Protein Name: L-lactate dehydrogenase A chain
• Protein Accession #: NP_005557
• Purification: Affinity Purified
• Nucleotide Accession #: NM_005566
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: LDHA
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Sheep
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 86%; Dog: 86%; Goat: 86%; Guinea Pig: 93%; Horse: 86%; Human: 100%; Mouse: 100%; Rabbit: 92%; Rat: 93%; Sheep: 86%
• Image 1: Human Ovary Tumor
  
  Host: Rabbit
  Target Name: LDHA
  Sample Tissue: Human Ovary Tumor
  Antibody Dilution: 1ug/ml
• Image 2: Human Liver
  
  Anti-LDHA antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. LDHA Antibody concentration 2.5 ug/ml.
• Image 3: Human A549 Whole Cell
  
  Host: Rabbit
  Target Name: LDHA
  Sample Tissue: Human A549 Whole Cell
  Antibody Dilution: 3ug/ml
• Image 4:
  
  Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.