| Product Number |
ARP54750_P050 |
| Product Page |
www.avivasysbio.com/grk5-antibody-middle-region-arp54750-p050.html |
| Name |
GRK5 Antibody - middle region (ARP54750_P050) |
| Protein Size (# AA) |
590 amino acids |
| Molecular Weight |
68kDa |
| NCBI Gene Id |
2869 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
G protein-coupled receptor kinase 5 |
| Description |
|
| Alias Symbols |
GPRK5, FP2025 |
| Peptide Sequence |
Synthetic peptide located within the following region: FYAAEILCGLEDLHRENTVYRDLKPENILLDDYGHIRISDLGLAVKIPEG |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Liggett,S.B., (2008) Nat. Med. 14 (5), 510-517 |
| Description of Target |
GRK5 is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. It has also been shown to play a role in regulating the motility of polymorphonuclear leukocytes (PMNs).This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. It has also been shown to play a role in regulating the motility of polymorphonuclear leukocytes (PMNs). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
| Protein Interactions |
TBXA2R; USP47; C1QBP; YWHAB; XRCC5; UBTF; UBC; TUFM; NR2F1; TCOF1; SRPK1; SPTBN1; SPTAN1; SMARCA1; SKP1; SRSF1; RPL4; RPL3; RBMS1; PSMD11; PSMD2; PSMC6; PSMC2; PSMC1; PRKDC; PPP2R2A; PNN; PIP4K2A; PIP; NFIB; NCL; NCBP1; MYO9B; MYO1C; MYH10; MYH9; MYCN; MA |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-GRK5 (ARP54750_P050) antibody |
| Blocking Peptide |
For anti-GRK5 (ARP54750_P050) antibody is Catalog # AAP54750 (Previous Catalog # AAPP31545) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human GRK5 |
| Uniprot ID |
P34947 |
| Protein Name |
G protein-coupled receptor kinase 5 |
| Publications |
The analysis of heterotaxy patients reveals new loss-of-function variants of GRK5. Sci Rep. 6, 33231 (2016). 27618959 |
| Protein Accession # |
NP_005299 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_005308 |
| Tested Species Reactivity |
Human, Mouse |
| Gene Symbol |
GRK5 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish |
| Application |
WB, IHC |
| Predicted Homology Based on Immunogen Sequence |
Cow: 86%; Dog: 100%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 100%; Rabbit: 86%; Rat: 100%; Yeast: 79%; Zebrafish: 86% |
| Image 1 | Human Lung Tissue
 | GRK5 antibody - middle region (ARP54750_P050)
Catalog Number: ARP54750_P050
Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue
Observed Staining: Cytoplasm and membrane of alveolar macrophages
Primary Antibody Concentration: 1:100
Other Working Concentrations: 1/600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec |
|
| Image 2 | mouse left ventricle heart
 | Sample Type:
Lane 1: 20ug mouse left ventricle heart lysate Primary Antibody Dilution:
1:1000 Secondary Antibody:
Anti-rabbit-HRP Secondary Antibody Dilution:
1:5000 Color/Signal Descriptions:
GRK5 Gene Name:
Kathleen Gabrielson Submitted by:
|
|
| Image 3 | Mouse Spleen
 | Host: Mouse
Target Name: GRK5
Sample Tissue: Mouse Spleen
Antibody Dilution: 1ug/ml |
|
| Image 4 | Human Brain
 | WB Suggested Anti-GRK5 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Human brain |
|
| Image 5 | Human GRK5, Rat striatum
 | Lanes:
Lane1: 1ug human GRK5 protein
Lane2: 0.25ug human GRK5 protein
Lane3: 0.1ug human GRK5 protein
Lane4: 5ug rat striatum homogenate
Lane5: 5ug rat striatum cytosol
Lane6: 5ug rat striatum light membrane fraction
Lane7: 5ug rat striatum synaptic membrane fraction
Lane8: 5ug rat striatum crude synaptic vesicle fraction
Lane9: 5ug rat striatum homogenate
Lane10: 5ug rat striatum cytosol
Lane11: 5ug rat striatum light membrane fraction
Lane12: 5ug rat striatum synaptic membrane fraction
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-rabbit HRP
Secondary Antibody Dilution:
1:15000
Gene Name:
GRK5
Submitted by:
Eugenia V Gurevich, Vanderbilt University
|
|
| Image 6 | tobacco hornworm
 | Lanes:
Lanes 1-3: 40 ug tobacco hornworm larvae intestine lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Goat anti-rabbit HRP
Secondary Antibody Dilution:
1:10000
Gene Name:
GRK5
Submitted by:
Dra. Helena Porta Ducoing and Biol. Gladys Jimenez Nopala
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