FLII Antibody - middle region (ARP54614_P050)

Data Sheet
 
Product Number ARP54614_P050
Product Page www.avivasysbio.com/flii-antibody-middle-region-arp54614-p050.html
Name FLII Antibody - middle region (ARP54614_P050)
Protein Size (# AA) 1269 amino acids
Molecular Weight 145kDa
NCBI Gene Id 2314
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Flightless I homolog (Drosophila)
Alias Symbols FLI, FLIL, Fli1
Peptide Sequence Synthetic peptide located within the following region: LAEDILNTMFDTSYSKQVINEGEEPENFFWVGIGAQKPYDDDAEYMKHTR
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Olsen,J.V., (2006) Cell 127 (3), 635-648
Description of Target FLII is a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. This gene is located within the Smith-Magenis syndrome region on chromosome 17.This gene encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Protein Interactions UBC; SUMO2; C14orf166; RTCB; IGF2BP3; PDCD6; LRRFIP1; EIF2B3; RPL27; RFC4; RFC2; PRKDC; NMT1; HNRNPM; MRE11A; KRT18; DHX9; DDX1; PAN2; HSP90AA1; FMNL1; BRCA1; EIF1AX; DGCR8; FBXO25; LRRFIP2; MYD88; ESR1; ACTL6A; GRIP1; CARM1; TRAIP; PRMT1; OPTN; DLST; ACT
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-FLII (ARP54614_P050) antibody
Blocking Peptide For anti-FLII (ARP54614_P050) antibody is Catalog # AAP54614 (Previous Catalog # AAPP31405)
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human FLII
Uniprot ID Q13045
Protein Name Protein flightless-1 homolog
Protein Accession # NP_002009
Purification Affinity Purified
Nucleotide Accession # NM_002018
Tested Species Reactivity Human
Gene Symbol FLII
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish
Application WB, IHC
Predicted Homology Based on Immunogen Sequence Cow: 100%; Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 79%
Image 1
Human OVCAR-3
WB Suggested Anti-FLII Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: OVCAR-3 cell lysate
Image 2
Human Bronchial Epithelial Tissue
FLII antibody - middle region (ARP54614_P050)
Catalog Number: ARP54614_P050
Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue
Observed Staining: Cytoplasm and membrane of bronchial epithelial tissue
Primary Antibody Concentration: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
 

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