| Product Number |
ARP54577_P050 |
| Product Page |
www.avivasysbio.com/gclc-antibody-middle-region-arp54577-p050.html |
| Name |
GCLC Antibody - middle region (ARP54577_P050) |
| Protein Size (# AA) |
637 amino acids |
| Molecular Weight |
73 kDa |
| NCBI Gene Id |
2729 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Glutamate-cysteine ligase, catalytic subunit |
| Description |
|
| Alias Symbols |
GCL, GCS, GLCL, GLCLC |
| Peptide Sequence |
Synthetic peptide located within the following region: RISKSRYDSIDSYLSKCGEKYNDIDLTIDKEIYEQLLQEGIDHLLAQHVA |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Description of Target |
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. GCLC is the catalytic subunit of 637 amino acids with a calculated molecular weight of 72.773 kDa. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia. |
| Protein Interactions |
UBC; PPID; GCLM; CCL22; PAXIP1; ELAVL1; |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Enhanced Validation |
|
| Datasheets/Manuals |
Printable datasheet for anti-GCLC (ARP54577_P050) antibody |
| Blocking Peptide |
For anti-GCLC (ARP54577_P050) antibody is Catalog # AAP54577 (Previous Catalog # AAPP44516) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human GCLC |
| Uniprot ID |
P48506 |
| Protein Name |
Glutamate--cysteine ligase catalytic subunit |
| Publications |
Bardag-Gorce, F. et al. Proteasome inhibitor up regulates liver antioxidative enzymes in rat model of alcoholic liver disease. Exp. Mol. Pathol. 90, 123-30 (2011). 21036165
Ramani, K., Tomasi, M. L., Yang, H., Ko, K. & Lu, S. C. Mechanism and significance of changes in glutamate-cysteine ligase expression during hepatic fibrogenesis. J. Biol. Chem. 287, 36341-55 (2012). 22942279
Saturated Fatty Acid Activates T Cell Inflammation Through a Nicotinamide Nucleotide Transhydrogenase (NNT)-Dependent Mechanism. Biomolecules. 9, (2019). 30823587 |
| Protein Accession # |
NP_001489 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_001498 |
| Tested Species Reactivity |
Human, Mouse |
| Gene Symbol |
GCLC |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish |
| Application |
IHC, WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 100%; Zebrafish: 100% |
| Image 1 | Human Placenta
 | WB Suggested Anti-GCLC Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: Human Placenta |
|
| Image 2 | Human THP-1 Whole Cell
 | Host: Rabbit
Target Name: GCLC
Sample Tissue: Human THP-1 Whole Cell
Antibody Dilution: 3ug/ml |
|
