GSR Antibody - N-terminal region (ARP54344_P050)

Data Sheet
 
Product Number ARP54344_P050
Product Page www.avivasysbio.com/gsr-antibody-n-terminal-region-arp54344-p050.html
Name GSR Antibody - N-terminal region (ARP54344_P050)
Protein Size (# AA) 522 amino acids
Molecular Weight 56kDa
NCBI Gene Id 2936
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Glutathione reductase
Alias Symbols GR, GSRD, HEL-75, HEL-S-122m
Peptide Sequence Synthetic peptide located within the following region: PTIEVSGKKYTAPHILIATGGMPSTPHESQIPGASLGITSDGFFQLEELP
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Senturk,M., (2008) J Enzyme Inhib Med Chem 23 (1), 144-148
Description of Target GSR belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. It maintains high levels of reduced glutathione in the cytosol. Both glutathione and glutathione reductase are inducible by D3T, and that upregulation of GSH biosynthesis underlies D3T-mediated cytoprotection against ROS/RNS-elicited injury to human vascular smooth muscle cells.
Protein Interactions SUMO1; CNDP2; GINS2; PROSC; AHSA1; NDRG1; DDX39A; NAMPT; KYNU; DDX39B; RPS6KA1; PEPD; MVD; LDHA; HSPD1; GBP2; EEF2; ADSS; C11orf49; UBC; FOLR1; ISG15; ELAVL1; SUMO4; PJA1; OCM; GSR;
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-GSR (ARP54344_P050) antibody
Blocking Peptide For anti-GSR (ARP54344_P050) antibody is Catalog # AAP54344 (Previous Catalog # AAPP31092)
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human GSR
Uniprot ID P00390
Protein Name Glutathione reductase, mitochondrial
Sample Type Confirmation

GSR is supported by BioGPS gene expression data to be expressed in 721_B, HepG2

Protein Accession # NP_000628
Purification Affinity Purified
Nucleotide Accession # NM_000637
Tested Species Reactivity Human, Mouse, Zebrafish
Gene Symbol GSR
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
Application IHC, WB
Predicted Homology Based on Immunogen Sequence Cow: 93%; Dog: 93%; Guinea Pig: 100%; Horse: 86%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 93%
Image 1
zebrafish head
WB Suggested Anti-GSR Antibody
Positive Control: Lane 1: 10ug untreated zebrafish head lysate Lane 2: 10ug 3hr H2O2 treated zebrafish head lysate Lane 3: 10ug 6hr H2O2 treated zebrafish head lysate
Primary Antibody Dilution : 1:1000
Secondary Antibody : Anti rabbit-HRP
Secondry Antibody Dilution : 1:10,000
Submitted by: Dr. Yuk Fai Leung, Purdue University
Image 2
Human Fetal Liver
Host: Rabbit
Target Name: GSR
Sample Tissue: Human Fetal Liver
Antibody Dilution: 1.0ug/ml
Image 3
Mouse heart, mouse skeletal muscle
Lanes:
1: 40ug mouse heart lysate, 2: 40ug mouse skeletal muscle lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-rabbit HRP
Secondary Antibody Dilution:
1:10000
Gene Name:
GSR
Submitted by:
Anonymous
Image 4
Human Bronchial Epithelial Tissue
Rabbit Anti-GSR Antibody
Catalog Number: ARP54344_P050
Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue
Observed Staining: Cytoplasmic
Primary Antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
 

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