Product Number |
ARP54344_P050 |
Product Page |
www.avivasysbio.com/gsr-antibody-n-terminal-region-arp54344-p050.html |
Name |
GSR Antibody - N-terminal region (ARP54344_P050) |
Protein Size (# AA) |
522 amino acids |
Molecular Weight |
56kDa |
NCBI Gene Id |
2936 |
Host |
Rabbit |
Clonality |
Polyclonal |
Concentration |
0.5 mg/ml |
Gene Full Name |
Glutathione reductase |
Alias Symbols |
GR, GSRD, HEL-75, HEL-S-122m |
Peptide Sequence |
Synthetic peptide located within the following region: PTIEVSGKKYTAPHILIATGGMPSTPHESQIPGASLGITSDGFFQLEELP |
Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Reference |
Senturk,M., (2008) J Enzyme Inhib Med Chem 23 (1), 144-148 |
Description of Target |
GSR belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. It maintains high levels of reduced glutathione in the cytosol. Both glutathione and glutathione reductase are inducible by D3T, and that upregulation of GSH biosynthesis underlies D3T-mediated cytoprotection against ROS/RNS-elicited injury to human vascular smooth muscle cells. |
Protein Interactions |
SUMO1; CNDP2; GINS2; PROSC; AHSA1; NDRG1; DDX39A; NAMPT; KYNU; DDX39B; RPS6KA1; PEPD; MVD; LDHA; HSPD1; GBP2; EEF2; ADSS; C11orf49; UBC; FOLR1; ISG15; ELAVL1; SUMO4; PJA1; OCM; GSR; |
Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Datasheets/Manuals |
Printable datasheet for anti-GSR (ARP54344_P050) antibody |
Blocking Peptide |
For anti-GSR (ARP54344_P050) antibody is Catalog # AAP54344 (Previous Catalog # AAPP31092) |
Immunogen |
The immunogen is a synthetic peptide directed towards the N terminal region of human GSR |
Uniprot ID |
P00390 |
Protein Name |
Glutathione reductase, mitochondrial |
Sample Type Confirmation |
GSR is supported by BioGPS gene expression data to be expressed in 721_B, HepG2 |
Protein Accession # |
NP_000628 |
Purification |
Affinity Purified |
Nucleotide Accession # |
NM_000637 |
Tested Species Reactivity |
Human, Mouse, Zebrafish |
Gene Symbol |
GSR |
Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit |
Application |
IHC, WB |
Predicted Homology Based on Immunogen Sequence |
Cow: 93%; Dog: 93%; Guinea Pig: 100%; Horse: 86%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 93% |
Image 1 | zebrafish head
| WB Suggested Anti-GSR Antibody Positive Control: Lane 1: 10ug untreated zebrafish head lysate Lane 2: 10ug 3hr H2O2 treated zebrafish head lysate Lane 3: 10ug 6hr H2O2 treated zebrafish head lysate Primary Antibody Dilution : 1:1000 Secondary Antibody : Anti rabbit-HRP Secondry Antibody Dilution : 1:10,000 Submitted by: Dr. Yuk Fai Leung, Purdue University |
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Image 2 | Human Fetal Liver
| Host: Rabbit Target Name: GSR Sample Tissue: Human Fetal Liver Antibody Dilution: 1.0ug/ml |
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Image 3 | Mouse heart, mouse skeletal muscle
| Lanes: 1: 40ug mouse heart lysate, 2: 40ug mouse skeletal muscle lysate Primary Antibody Dilution: 1:1000 Secondary Antibody: Anti-rabbit HRP Secondary Antibody Dilution: 1:10000 Gene Name: GSR Submitted by: Anonymous
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Image 4 | Human Bronchial Epithelial Tissue
| Rabbit Anti-GSR Antibody Catalog Number: ARP54344_P050 Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue Observed Staining: Cytoplasmic Primary Antibody Concentration: 1:100 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1:200 Magnification: 20X Exposure Time: 0.5 - 2.0 sec
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