| Product Number |
ARP53652_P050 |
| Product Page |
www.avivasysbio.com/prkaa1-antibody-middle-region-arp53652-p050.html |
| Name |
PRKAA1 Antibody - middle region (ARP53652_P050) |
| Protein Size (# AA) |
559 amino acids |
| Molecular Weight |
63kDa |
| Subunit |
alpha-1 |
| NCBI Gene Id |
5562 |
| Host |
Rabbit |
| Clonality |
Polyclonal |
| Concentration |
0.5 mg/ml |
| Gene Full Name |
Protein kinase, AMP-activated, alpha 1 catalytic subunit |
| Alias Symbols |
AMPK, AMPKa1, AMPK alpha 1 |
| Peptide Sequence |
Synthetic peptide located within the following region: SVISLLKHMLQVDPMKRATIKDIREHEWFKQDLPKYLFPEDPSYSSTMID |
| Product Format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
| Reference |
Hasumi,H., Gene 415 (1-2), 60-67 (2008) |
| Description of Target |
PRKAA1 belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. |
| Protein Interactions |
PRKAG1; PRKAB2; PRKAB1; GOLGA2; PHC2; CTBP1; CDX4; AES; Trp53; INO80E; RFX6; TRIM27; KRT40; SSX2IP; PNMA5; UBXN11; RIMBP3; L3MBTL3; TSC22D4; VPS37B; HMBOX1; HOMEZ; THAP1; ROPN1; RAD54B; MTUS2; IKZF3; RBPMS; ABI2; ZBED1; BHLHE40; TRIP6; STK11; VPS52; PPARG |
| Reconstitution and Storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Datasheets/Manuals |
Printable datasheet for anti-PRKAA1 (ARP53652_P050) antibody |
| Blocking Peptide |
For anti-PRKAA1 (ARP53652_P050) antibody is Catalog # AAP53652 (Previous Catalog # AAPP30492) |
| Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human PRKAA1 |
| Uniprot ID |
Q13131 |
| Protein Name |
5'-AMP-activated protein kinase catalytic subunit alpha-1 |
| Protein Accession # |
NP_006242 |
| Purification |
Affinity Purified |
| Nucleotide Accession # |
NM_006251 |
| Tested Species Reactivity |
Human, Mouse, Rat |
| Gene Symbol |
PRKAA1 |
| Predicted Species Reactivity |
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish |
| Application |
WB |
| Predicted Homology Based on Immunogen Sequence |
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Sheep: 93%; Zebrafish: 100% |
| Image 1 | Human COLO205
 | WB Suggested Anti-PRKAA1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: COLO205 cell lysate |
|
| Image 2 | Rat hepatocyte
 | Sample Type: rat hepatocyte (50ug)
Priamry Dilution: 1:4000
Secondary Antibody: Donkey anti-Rabbit HRP
Secondary Dilution: 1:10,000
Image Submitted By: Dustin Singer,
INRA/AgroParistech |
|
| Image 3 | Rat Liver, Mouse muscle
 | PRKAA1 antibody - middle region (ARP53652_P050) validated by WB using Rat liver and Mouse muscle at 1:1000. |
|
