HSPA1L Antibody - C-terminal region (ARP53636_P050)

Data Sheet
 
Product Number ARP53636_P050
Product Page www.avivasysbio.com/hspa1l-antibody-c-terminal-region-arp53636-p050.html
Name HSPA1L Antibody - C-terminal region (ARP53636_P050)
Protein Size (# AA) 641 amino acids
Molecular Weight 70kDa
NCBI Gene Id 3305
Host Rabbit
Clonality Polyclonal
Concentration 0.5 mg/ml
Gene Full Name Heat shock 70kDa protein 1-like
Alias Symbols HSP70T, hum70t, HSP70-1L, HSP70-HOM
Peptide Sequence Synthetic peptide located within the following region: DEFDHKRKELEQMCNPIITKLYQGGCTGPACGTGYVPGRPATGPTIEEVD
Product Format Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reference Buraczynska,M., (er) Clin. Sci. (2008) In press
Description of Target HSPA1L is a 70kDa heat shock protein. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. This gene encodes a 70kDa heat shock protein. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which also encode isoforms of the 70kDa heat shock protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Protein Interactions TUBG1; TP53; CDC20; AIRE; NEDD1; CEP250; CEP57; STUB1; BAG4; DNAJC7; STAU1; UBC; ZBTB1; TRIM25; XPO1; RAP1GDS1; NUCB2; HSPA5; GAPDH; CLU; CAMK1D; UBQLN1; IPO7; PARK2; BAG3; HDAC6; K5; METTL21C; DCP1B; METTL21A; CFTR; METTL22; DCP1A; METTL21B; UBL4A; TRAF3
Reconstitution and Storage For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for anti-HSPA1L (ARP53636_P050) antibody
Blocking Peptide For anti-HSPA1L (ARP53636_P050) antibody is Catalog # AAP53636 (Previous Catalog # AAPP30476)
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human HSPA1L
Uniprot ID P34931
Protein Name Heat shock 70 kDa protein 1-like
Protein Accession # NP_005518
Purification Affinity Purified
Nucleotide Accession # NM_005527
Tested Species Reactivity Human, Rat
Gene Symbol HSPA1L
Predicted Species Reactivity Human, Mouse, Rat, Cow, Dog, Goat, Horse, Pig
Application WB, IP
Predicted Homology Based on Immunogen Sequence Cow: 86%; Dog: 86%; Goat: 86%; Horse: 86%; Human: 100%; Mouse: 86%; Pig: 86%; Rat: 86%
Image 1
Rat, Lamprey
Sample Type: 1. Lamprey CNS (20ug)
2. Rat Brain Lysate (20ug) 
Primary Dilution: 1:1000
Secondary Antibody: Goat anti-Rabbit HRP
Secondary Dilution: 1:4000
Image Submitted by: Jennifer Morgan, PhD & David Busch
University of Texas in Austin 
Image 2
Human MDA-MB-435s
Host: Rabbit
Target Name: HSPA1L
Sample Tissue: Human MDA-MB-435s
Antibody Dilution: 1.0ug/ml
Image 3
Human Testis
Rabbit Anti-HSPA1L antibody
Catalog Number: ARP53636
Formalin Fixed Paraffin Embedded Tissue: Human Testis
Primary antibody Concentration: 1:200
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20x
Exposure Time: 0.5-2.0sec
Image 4

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 0.2 ug/mL of the antibody was used in this experiment.
 

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