DNM1L Antibody - N-terminal region (ARP52329_P050)

• Product Number: ARP52329_P050
• Name: DNM1L Antibody - N-terminal region (ARP52329_P050)
• Protein Size (# AA): 736 amino acids
• Molecular Weight: 82 kDa
• NCBI Gene Id: 10059
• Host: Rabbit
• Clonality: Polyclonal
• Concentration: 0.5 mg/ml
• Gene Full Name: Dynamin 1-like
• Alias Symbols: DLP1, DRP1, DVLP, EMPF, OPA5, EMPF1, DYMPLE, HDYNIV
• Peptide Sequence: Synthetic peptide located within the following region: IRQEIENETERISGNNKGVSPEPIHLKIFSPNVVNLTLVDLPGMTKVPVG
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Description of Target: The protein encoded by this gene is a member of the dynamin superfamily of GTPases. Members of the dynamin-related subfamily, including the S. cerevisiae proteins Dnm1 and Vps1, contain the N-terminal tripartite GTPase domain but do not have the pleckstrin homology or proline-rich domains. This protein establishes mitochondrial morphology through a role in distributing mitochondrial tubules throughout the cytoplasm. The gene has 3 alternatively spliced transcripts encoding different isoforms. These transcripts are alternatively polyadenylated.
• Protein Interactions: ZBTB24; UBC; SCG3; SH3GL1; RRM2; MAGEA3; MAGEA1; MARCH5; EGFR; VPS26A; SSB; CARS; ATP6V1C1; APEH; PSMG3; FAM129B; TBC1D15; CYHR1; ARFIP1; JMJD6; SEC24A; SOX2; DNM1L; MIEF2; MFF; PEX14; LRRK2; MIEF1; COPS5; GABRR1; MUL1; SUMO2; PARK2; PEX25; PEX11G; PEX11A
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Enhanced Validation:

  WB	Y
  SPR
  YCHAROS

• Blocking Peptide: For anti-DNM1L (ARP52329_P050) antibody is Catalog # AAP52329 (Previous Catalog # AAPP42835)
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DNM1L
• Uniprot ID: O00429
• Protein Name: Dynamin-1-like protein
• Publications:

  Su, Y. C., Chiu, H. W., Hung, J. C. & Hong, J. R. Beta-nodavirus B2 protein induces hydrogen peroxide production, leading to Drp1-recruited mitochondrial fragmentation and cell death via mitochondrial targeting. Apoptosis 19, 1457-70 (2014). 25008790

• Sample Type Confirmation:

  DNM1L is supported by BioGPS gene expression data to be expressed in HeLa

• Protein Accession #: NP_036192
• Purification: Affinity Purified
• Nucleotide Accession #: NM_012062
• Tested Species Reactivity: Human, Mouse
• Gene Symbol: DNM1L
• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish
• Application: IHC, WB
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 85%; Zebrafish: 93%
• Image 1: Human HeLa
  
  WB Suggested Anti-DNM1L Antibody Titration: 0.2-1 ug/ml
  Positive Control: Hela cell lysateDNM1L is supported by BioGPS gene expression data to be expressed in HeLa
• Image 2: A549, HepG2
  
  Host: Rabbit
  Target: DNM1L
  Positive control (+): A549 (N03)
  Negative control (-): HepG2 (HG)
  Antibody concentration: 3ug/ml
• Image 3: Mouse Brain
  
  Researcher: Dr. Yuzhi Chen, University of Arkansas for Medical Science
  Application: IHC
  Species+tissue/cell type: Mouse brain stem cells
  Primary antibody dilution: 1:500
  Secondary antibody: Goat anti-rabbit Alexa-Fluor 594
  Secondary antibody dilution: 1:1000
• Image 4: Human DLD1 Whole Cell
  
  Host: Rabbit
  Target Name: DNM1L
  Sample Tissue: Human DLD1 Whole Cell
  Antibody Dilution: 1ug/ml